<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2024 Workiva-->
<!--r:d1cdf21e-70ae-4665-ab94-12b7d9b08d05,g:3c89a432-fcfd-4249-8f55-5ec6fe25a53a-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/AcquisitionsandDivestituresDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#AcquisitionsandDivestituresDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/AcquisitionsandDivestituresDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsLineItems_600609e8-bcec-4b56-bc4b-9cb9de90d99a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfBusinessesAcquired_aad5a686-ce0c-41fa-ac3b-e771f9aa390d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NumberOfBusinessesAcquired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsLineItems_600609e8-bcec-4b56-bc4b-9cb9de90d99a" xlink:to="loc_us-gaap_NumberOfBusinessesAcquired_aad5a686-ce0c-41fa-ac3b-e771f9aa390d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_DisposalGroupIncludingDiscontinuedOperationNumberOfDivestitures_fe5288d5-cb2d-442b-8d70-7c79142f9018" xlink:href="ayi-20231130.xsd#ayi_DisposalGroupIncludingDiscontinuedOperationNumberOfDivestitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsLineItems_600609e8-bcec-4b56-bc4b-9cb9de90d99a" xlink:to="loc_ayi_DisposalGroupIncludingDiscontinuedOperationNumberOfDivestitures_fe5288d5-cb2d-442b-8d70-7c79142f9018" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_803d206e-8888-4b4d-a617-d031ff3f5736" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsLineItems_600609e8-bcec-4b56-bc4b-9cb9de90d99a" xlink:to="loc_us-gaap_Goodwill_803d206e-8888-4b4d-a617-d031ff3f5736" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillAcquiredDuringPeriod_6343fdff-ee2c-4559-adb3-0979ff9fbc5a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GoodwillAcquiredDuringPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsLineItems_600609e8-bcec-4b56-bc4b-9cb9de90d99a" xlink:to="loc_us-gaap_GoodwillAcquiredDuringPeriod_6343fdff-ee2c-4559-adb3-0979ff9fbc5a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill_aee49926-1b97-4047-8713-a81540c57e0c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsLineItems_600609e8-bcec-4b56-bc4b-9cb9de90d99a" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill_aee49926-1b97-4047-8713-a81540c57e0c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsWeightedAverageUsefulLife_ffa34b88-1f58-4977-bee8-9de6c132ec32" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AcquiredFiniteLivedIntangibleAssetsWeightedAverageUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsLineItems_600609e8-bcec-4b56-bc4b-9cb9de90d99a" xlink:to="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsWeightedAverageUsefulLife_ffa34b88-1f58-4977-bee8-9de6c132ec32" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsOfDisposalGroupIncludingDiscontinuedOperationCurrent_ffd4fbc5-9f3f-4334-b097-8c3240f6cf2a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AssetsOfDisposalGroupIncludingDiscontinuedOperationCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsLineItems_600609e8-bcec-4b56-bc4b-9cb9de90d99a" xlink:to="loc_us-gaap_AssetsOfDisposalGroupIncludingDiscontinuedOperationCurrent_ffd4fbc5-9f3f-4334-b097-8c3240f6cf2a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisposalGroupNotDiscontinuedOperationGainLossOnDisposal_2203728f-12ec-4003-ab4c-c7794404c783" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisposalGroupNotDiscontinuedOperationGainLossOnDisposal"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsLineItems_600609e8-bcec-4b56-bc4b-9cb9de90d99a" xlink:to="loc_us-gaap_DisposalGroupNotDiscontinuedOperationGainLossOnDisposal_2203728f-12ec-4003-ab4c-c7794404c783" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsTable_871c290c-0718-44fd-b29b-565bc52f3db0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsLineItems_600609e8-bcec-4b56-bc4b-9cb9de90d99a" xlink:to="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsTable_871c290c-0718-44fd-b29b-565bc52f3db0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsAxis_96a8993a-443a-4830-8748-55c41ea72ab1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsTable_871c290c-0718-44fd-b29b-565bc52f3db0" xlink:to="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsAxis_96a8993a-443a-4830-8748-55c41ea72ab1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain_96a8993a-443a-4830-8748-55c41ea72ab1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsAxis_96a8993a-443a-4830-8748-55c41ea72ab1" xlink:to="loc_us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain_96a8993a-443a-4830-8748-55c41ea72ab1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain_b857b56e-ffa9-4cf7-a804-1b2aa1e8477c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsAxis_96a8993a-443a-4830-8748-55c41ea72ab1" xlink:to="loc_us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain_b857b56e-ffa9-4cf7-a804-1b2aa1e8477c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_SunopticsBusinessMember_65764876-b7b3-4de4-9f7b-157f68f5fc5b" xlink:href="ayi-20231130.xsd#ayi_SunopticsBusinessMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisposalGroupsIncludingDiscontinuedOperationsNameDomain_b857b56e-ffa9-4cf7-a804-1b2aa1e8477c" xlink:to="loc_ayi_SunopticsBusinessMember_65764876-b7b3-4de4-9f7b-157f68f5fc5b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_a54f27fb-87b6-4a90-bce8-c63aa8f82cfa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsTable_871c290c-0718-44fd-b29b-565bc52f3db0" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_a54f27fb-87b6-4a90-bce8-c63aa8f82cfa" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_a54f27fb-87b6-4a90-bce8-c63aa8f82cfa_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_a54f27fb-87b6-4a90-bce8-c63aa8f82cfa" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_a54f27fb-87b6-4a90-bce8-c63aa8f82cfa_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_138a553e-00e1-4cce-8f4e-08c5387d83ba" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_a54f27fb-87b6-4a90-bce8-c63aa8f82cfa" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_138a553e-00e1-4cce-8f4e-08c5387d83ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_KE2ThermSolutionsIncMember_5e5dbdfc-475c-4c72-a869-a250b521af91" xlink:href="ayi-20231130.xsd#ayi_KE2ThermSolutionsIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_138a553e-00e1-4cce-8f4e-08c5387d83ba" xlink:to="loc_ayi_KE2ThermSolutionsIncMember_5e5dbdfc-475c-4c72-a869-a250b521af91" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_05110af1-82b4-4738-9ad5-eac1b3489264" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsTable_871c290c-0718-44fd-b29b-565bc52f3db0" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_05110af1-82b4-4738-9ad5-eac1b3489264" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_05110af1-82b4-4738-9ad5-eac1b3489264_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_05110af1-82b4-4738-9ad5-eac1b3489264" xlink:to="loc_us-gaap_SegmentDomain_05110af1-82b4-4738-9ad5-eac1b3489264_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_fac913d2-b25a-45df-8905-87e161d3f325" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_05110af1-82b4-4738-9ad5-eac1b3489264" xlink:to="loc_us-gaap_SegmentDomain_fac913d2-b25a-45df-8905-87e161d3f325" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_IntelligentSpacesGroupSegmentMember_f76e3bd2-943c-419a-b21e-98e5d8390d89" xlink:href="ayi-20231130.xsd#ayi_IntelligentSpacesGroupSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_fac913d2-b25a-45df-8905-87e161d3f325" xlink:to="loc_ayi_IntelligentSpacesGroupSegmentMember_f76e3bd2-943c-419a-b21e-98e5d8390d89" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisposalGroupClassificationAxis_cc8dbec8-fab0-4a4e-bb10-9de04268a527" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisposalGroupClassificationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_IncomeStatementBalanceSheetAndAdditionalDisclosuresByDisposalGroupsIncludingDiscontinuedOperationsTable_871c290c-0718-44fd-b29b-565bc52f3db0" xlink:to="loc_us-gaap_DisposalGroupClassificationAxis_cc8dbec8-fab0-4a4e-bb10-9de04268a527" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisposalGroupClassificationDomain_cc8dbec8-fab0-4a4e-bb10-9de04268a527_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisposalGroupClassificationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DisposalGroupClassificationAxis_cc8dbec8-fab0-4a4e-bb10-9de04268a527" xlink:to="loc_us-gaap_DisposalGroupClassificationDomain_cc8dbec8-fab0-4a4e-bb10-9de04268a527_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisposalGroupClassificationDomain_7cbb50e2-c698-489c-81da-77798576d62d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisposalGroupClassificationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DisposalGroupClassificationAxis_cc8dbec8-fab0-4a4e-bb10-9de04268a527" xlink:to="loc_us-gaap_DisposalGroupClassificationDomain_7cbb50e2-c698-489c-81da-77798576d62d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisposalGroupNotDiscontinuedOperationsMember_db05d2dc-518b-4918-be2f-83e24b0d2ebb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisposalGroupNotDiscontinuedOperationsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisposalGroupClassificationDomain_7cbb50e2-c698-489c-81da-77798576d62d" xlink:to="loc_us-gaap_DisposalGroupNotDiscontinuedOperationsMember_db05d2dc-518b-4918-be2f-83e24b0d2ebb" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/FairValueMeasurementsScheduleofCarryingValueandEstimatedFairValueofFinancialInstrumentsDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#FairValueMeasurementsScheduleofCarryingValueandEstimatedFairValueofFinancialInstrumentsDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/FairValueMeasurementsScheduleofCarryingValueandEstimatedFairValueofFinancialInstrumentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_a1e21d1a-8770-41da-88cc-d7fcc9afb10d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_ec0ae8aa-7477-4f71-b78c-722e1efb87a0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_a1e21d1a-8770-41da-88cc-d7fcc9afb10d" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_ec0ae8aa-7477-4f71-b78c-722e1efb87a0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsFairValueDisclosure_33129452-b1da-400c-894e-b16ce5b31c8c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherAssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_a1e21d1a-8770-41da-88cc-d7fcc9afb10d" xlink:to="loc_us-gaap_OtherAssetsFairValueDisclosure_33129452-b1da-400c-894e-b16ce5b31c8c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_465f02d2-1c3b-4bd1-b9a1-9d4d4eb0ffa1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_a1e21d1a-8770-41da-88cc-d7fcc9afb10d" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_465f02d2-1c3b-4bd1-b9a1-9d4d4eb0ffa1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesWithoutReadilyDeterminableFairValueAmount_e8fd4e99-3735-4477-acc3-25c6f380162e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquitySecuritiesWithoutReadilyDeterminableFairValueAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_a1e21d1a-8770-41da-88cc-d7fcc9afb10d" xlink:to="loc_us-gaap_EquitySecuritiesWithoutReadilyDeterminableFairValueAmount_e8fd4e99-3735-4477-acc3-25c6f380162e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AssetFairValueDisclosureIncludingEquitySecuritiesWithoutReadilyDeterminableFairValueAmount_5b6273f2-59bf-4127-98fc-eb1cf9592088" xlink:href="ayi-20231130.xsd#ayi_AssetFairValueDisclosureIncludingEquitySecuritiesWithoutReadilyDeterminableFairValueAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_a1e21d1a-8770-41da-88cc-d7fcc9afb10d" xlink:to="loc_ayi_AssetFairValueDisclosureIncludingEquitySecuritiesWithoutReadilyDeterminableFairValueAmount_5b6273f2-59bf-4127-98fc-eb1cf9592088" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_e12bfc03-8473-4d6e-aeec-bce3cfd9ada3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_a1e21d1a-8770-41da-88cc-d7fcc9afb10d" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_e12bfc03-8473-4d6e-aeec-bce3cfd9ada3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_cd0a4f8c-946e-4fb4-be14-54d98e1dda89" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_e12bfc03-8473-4d6e-aeec-bce3cfd9ada3" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_cd0a4f8c-946e-4fb4-be14-54d98e1dda89" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_cd0a4f8c-946e-4fb4-be14-54d98e1dda89_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_cd0a4f8c-946e-4fb4-be14-54d98e1dda89" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_cd0a4f8c-946e-4fb4-be14-54d98e1dda89_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_59511271-3de2-4299-97b9-1e641fecd2d8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_cd0a4f8c-946e-4fb4-be14-54d98e1dda89" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_59511271-3de2-4299-97b9-1e641fecd2d8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_f30032a7-b098-4e6d-9d9d-ffbf4191c847" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_59511271-3de2-4299-97b9-1e641fecd2d8" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_f30032a7-b098-4e6d-9d9d-ffbf4191c847" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_00c65d21-9144-4a09-8d43-b8609b8623b9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_e12bfc03-8473-4d6e-aeec-bce3cfd9ada3" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_00c65d21-9144-4a09-8d43-b8609b8623b9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_00c65d21-9144-4a09-8d43-b8609b8623b9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_00c65d21-9144-4a09-8d43-b8609b8623b9" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_00c65d21-9144-4a09-8d43-b8609b8623b9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_23ab6199-7ca0-458a-8eb5-08c488cf6d6f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_00c65d21-9144-4a09-8d43-b8609b8623b9" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_23ab6199-7ca0-458a-8eb5-08c488cf6d6f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_af4624ac-5f9d-4e2d-ad48-304b529be6b5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_23ab6199-7ca0-458a-8eb5-08c488cf6d6f" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_af4624ac-5f9d-4e2d-ad48-304b529be6b5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_53f897c9-974a-4057-9e92-a9ae17c0538c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_23ab6199-7ca0-458a-8eb5-08c488cf6d6f" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_53f897c9-974a-4057-9e92-a9ae17c0538c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel12And3Member_58cd4f0f-0fdc-4c00-a6c1-083f35fe7c0b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueInputsLevel12And3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_23ab6199-7ca0-458a-8eb5-08c488cf6d6f" xlink:to="loc_us-gaap_FairValueInputsLevel12And3Member_58cd4f0f-0fdc-4c00-a6c1-083f35fe7c0b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasuredAtNetAssetValuePerShareMember_e91f5a4a-cca5-4840-ab99-eebf02dbdd44" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasuredAtNetAssetValuePerShareMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_23ab6199-7ca0-458a-8eb5-08c488cf6d6f" xlink:to="loc_us-gaap_FairValueMeasuredAtNetAssetValuePerShareMember_e91f5a4a-cca5-4840-ab99-eebf02dbdd44" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#FairValueMeasurementsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_e2c50f8d-8ff7-44fd-be84-d202e50a6a87" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtFairValue_6bdce634-467a-4eff-9b1a-d479bc9716ec" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermDebtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_e2c50f8d-8ff7-44fd-be84-d202e50a6a87" xlink:to="loc_us-gaap_LongTermDebtFairValue_6bdce634-467a-4eff-9b1a-d479bc9716ec" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermBorrowings_5493d0bd-a7af-4251-8618-0f3e99b25e32" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShortTermBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_e2c50f8d-8ff7-44fd-be84-d202e50a6a87" xlink:to="loc_us-gaap_ShortTermBorrowings_5493d0bd-a7af-4251-8618-0f3e99b25e32" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShorttermDebtFairValue_126a6ae6-cd8a-4ea8-b7ac-53f22ca6294c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShorttermDebtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_e2c50f8d-8ff7-44fd-be84-d202e50a6a87" xlink:to="loc_us-gaap_ShorttermDebtFairValue_126a6ae6-cd8a-4ea8-b7ac-53f22ca6294c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_3a72727a-1ad8-449b-a342-c77d5d545655" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_e2c50f8d-8ff7-44fd-be84-d202e50a6a87" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_3a72727a-1ad8-449b-a342-c77d5d545655" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_0fec39ef-7cf2-4e73-b8b3-3ead9e7a231d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_3a72727a-1ad8-449b-a342-c77d5d545655" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_0fec39ef-7cf2-4e73-b8b3-3ead9e7a231d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_0fec39ef-7cf2-4e73-b8b3-3ead9e7a231d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_0fec39ef-7cf2-4e73-b8b3-3ead9e7a231d" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_0fec39ef-7cf2-4e73-b8b3-3ead9e7a231d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_770330f0-50d1-42fc-846c-f71b8f16de27" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_0fec39ef-7cf2-4e73-b8b3-3ead9e7a231d" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_770330f0-50d1-42fc-846c-f71b8f16de27" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_c318c8ac-049a-4265-8129-feea9c6f35a4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_770330f0-50d1-42fc-846c-f71b8f16de27" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_c318c8ac-049a-4265-8129-feea9c6f35a4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_d6bf07a1-265b-47d1-a05e-d703d3da1287" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_c318c8ac-049a-4265-8129-feea9c6f35a4" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_d6bf07a1-265b-47d1-a05e-d703d3da1287" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_06df0c6a-0a78-4952-a56b-84d031965529" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_3a72727a-1ad8-449b-a342-c77d5d545655" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_06df0c6a-0a78-4952-a56b-84d031965529" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_06df0c6a-0a78-4952-a56b-84d031965529_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_06df0c6a-0a78-4952-a56b-84d031965529" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_06df0c6a-0a78-4952-a56b-84d031965529_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_eec7fd6c-eb5a-4e6e-b66f-86ec00336fda" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_06df0c6a-0a78-4952-a56b-84d031965529" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_eec7fd6c-eb5a-4e6e-b66f-86ec00336fda" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnsecuredDebtMember_014c99d6-5acc-401c-a800-38481e1e9a68" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UnsecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_eec7fd6c-eb5a-4e6e-b66f-86ec00336fda" xlink:to="loc_us-gaap_UnsecuredDebtMember_014c99d6-5acc-401c-a800-38481e1e9a68" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_0954014d-01c0-4544-9f16-15d6449e60c1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_eec7fd6c-eb5a-4e6e-b66f-86ec00336fda" xlink:to="loc_us-gaap_LineOfCreditMember_0954014d-01c0-4544-9f16-15d6449e60c1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_6b132e90-7e36-481c-a0ef-c66ee5479f6a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_3a72727a-1ad8-449b-a342-c77d5d545655" xlink:to="loc_us-gaap_CreditFacilityAxis_6b132e90-7e36-481c-a0ef-c66ee5479f6a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_6b132e90-7e36-481c-a0ef-c66ee5479f6a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_6b132e90-7e36-481c-a0ef-c66ee5479f6a" xlink:to="loc_us-gaap_CreditFacilityDomain_6b132e90-7e36-481c-a0ef-c66ee5479f6a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_1ca72ab2-a42f-4898-a6e0-95297eabeeb8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_6b132e90-7e36-481c-a0ef-c66ee5479f6a" xlink:to="loc_us-gaap_CreditFacilityDomain_1ca72ab2-a42f-4898-a6e0-95297eabeeb8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_e35a15c5-0444-4ca0-8492-08010af6edd4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_1ca72ab2-a42f-4898-a6e0-95297eabeeb8" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_e35a15c5-0444-4ca0-8492-08010af6edd4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_881bd0cd-d43f-42da-b394-1c97dc0389b6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_3a72727a-1ad8-449b-a342-c77d5d545655" xlink:to="loc_us-gaap_DebtInstrumentAxis_881bd0cd-d43f-42da-b394-1c97dc0389b6" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_881bd0cd-d43f-42da-b394-1c97dc0389b6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_881bd0cd-d43f-42da-b394-1c97dc0389b6" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_881bd0cd-d43f-42da-b394-1c97dc0389b6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_8071ccc0-e375-4527-be90-770a1c35fd7b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_881bd0cd-d43f-42da-b394-1c97dc0389b6" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_8071ccc0-e375-4527-be90-770a1c35fd7b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_A2150SeniorUnsecuredNotesDueDecember2030Member_45098dd5-8e2c-4f18-a3bd-8fa0b4f43767" xlink:href="ayi-20231130.xsd#ayi_A2150SeniorUnsecuredNotesDueDecember2030Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_8071ccc0-e375-4527-be90-770a1c35fd7b" xlink:to="loc_ayi_A2150SeniorUnsecuredNotesDueDecember2030Member_45098dd5-8e2c-4f18-a3bd-8fa0b4f43767" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_TheRevolvingCreditFacilityMember_0a4b052f-cc2a-41ee-b47a-375ba99530e9" xlink:href="ayi-20231130.xsd#ayi_TheRevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_8071ccc0-e375-4527-be90-770a1c35fd7b" xlink:to="loc_ayi_TheRevolvingCreditFacilityMember_0a4b052f-cc2a-41ee-b47a-375ba99530e9" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/PropertyPlantandEquipmentScheduleofPropertyPlantandEquipmentDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#PropertyPlantandEquipmentScheduleofPropertyPlantandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/PropertyPlantandEquipmentScheduleofPropertyPlantandEquipmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7b337867-97d1-4964-b486-6345bdbae92e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_8a789313-7390-4a98-ad6a-cf55d409a228" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7b337867-97d1-4964-b486-6345bdbae92e" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_8a789313-7390-4a98-ad6a-cf55d409a228" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_b45be1b2-cc4e-4874-a39f-91fa234ac908" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7b337867-97d1-4964-b486-6345bdbae92e" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_b45be1b2-cc4e-4874-a39f-91fa234ac908" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_4c46ce04-4e8e-4d0c-a2ce-9a77b44083ad" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7b337867-97d1-4964-b486-6345bdbae92e" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_4c46ce04-4e8e-4d0c-a2ce-9a77b44083ad" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_b15a37c8-6546-462e-852f-24f0422ea5d1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_7b337867-97d1-4964-b486-6345bdbae92e" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_b15a37c8-6546-462e-852f-24f0422ea5d1" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_4ac02c1d-957d-41c1-940e-d1f5a4c2089f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_b15a37c8-6546-462e-852f-24f0422ea5d1" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_4ac02c1d-957d-41c1-940e-d1f5a4c2089f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_4ac02c1d-957d-41c1-940e-d1f5a4c2089f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_4ac02c1d-957d-41c1-940e-d1f5a4c2089f" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_4ac02c1d-957d-41c1-940e-d1f5a4c2089f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c9742eab-6182-4049-bb81-e34a96b27d83" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_4ac02c1d-957d-41c1-940e-d1f5a4c2089f" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c9742eab-6182-4049-bb81-e34a96b27d83" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LandMember_49bb79ed-427d-40fd-9487-ee6c2b2e7863" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c9742eab-6182-4049-bb81-e34a96b27d83" xlink:to="loc_us-gaap_LandMember_49bb79ed-427d-40fd-9487-ee6c2b2e7863" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BuildingAndBuildingImprovementsMember_af95db4c-9ec3-446f-9221-2375ab24ae40" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BuildingAndBuildingImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c9742eab-6182-4049-bb81-e34a96b27d83" xlink:to="loc_us-gaap_BuildingAndBuildingImprovementsMember_af95db4c-9ec3-446f-9221-2375ab24ae40" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MachineryAndEquipmentMember_f96136c9-6a83-425f-93db-5cf05d94e67c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_MachineryAndEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_c9742eab-6182-4049-bb81-e34a96b27d83" xlink:to="loc_us-gaap_MachineryAndEquipmentMember_f96136c9-6a83-425f-93db-5cf05d94e67c" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/GoodwillandIntangibleAssetsChangesinGoodwillDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#GoodwillandIntangibleAssetsChangesinGoodwillDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/GoodwillandIntangibleAssetsChangesinGoodwillDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_6b2bd5f6-86a1-449c-812c-e604b58a4c91" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillRollForward_afe7240b-5cb1-4a67-9597-6fbbd351625c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GoodwillRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_6b2bd5f6-86a1-449c-812c-e604b58a4c91" xlink:to="loc_us-gaap_GoodwillRollForward_afe7240b-5cb1-4a67-9597-6fbbd351625c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_4892d2ea-de2b-4d70-adee-164ba97577ab" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_afe7240b-5cb1-4a67-9597-6fbbd351625c" xlink:to="loc_us-gaap_Goodwill_4892d2ea-de2b-4d70-adee-164ba97577ab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillWrittenOffRelatedToSaleOfBusinessUnit_f466170c-b0cd-42b4-9bc6-7c03731eb021" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GoodwillWrittenOffRelatedToSaleOfBusinessUnit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_afe7240b-5cb1-4a67-9597-6fbbd351625c" xlink:to="loc_us-gaap_GoodwillWrittenOffRelatedToSaleOfBusinessUnit_f466170c-b0cd-42b4-9bc6-7c03731eb021" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillForeignCurrencyTranslationGainLoss_33035585-4cd1-47a9-adba-bfecda57ec59" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GoodwillForeignCurrencyTranslationGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_afe7240b-5cb1-4a67-9597-6fbbd351625c" xlink:to="loc_us-gaap_GoodwillForeignCurrencyTranslationGainLoss_33035585-4cd1-47a9-adba-bfecda57ec59" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_8357a9f8-2146-4587-afbe-76afd6bcf142" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Goodwill"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable_fcdd8dbc-b4ad-4526-a0bc-e13e76b6e42f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_6b2bd5f6-86a1-449c-812c-e604b58a4c91" xlink:to="loc_us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable_fcdd8dbc-b4ad-4526-a0bc-e13e76b6e42f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_f89412d7-c975-4e45-b442-024c8a9c88b2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable_fcdd8dbc-b4ad-4526-a0bc-e13e76b6e42f" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_f89412d7-c975-4e45-b442-024c8a9c88b2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_f89412d7-c975-4e45-b442-024c8a9c88b2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_f89412d7-c975-4e45-b442-024c8a9c88b2" xlink:to="loc_us-gaap_SegmentDomain_f89412d7-c975-4e45-b442-024c8a9c88b2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_7df33ddb-71c4-4e10-b7b7-99301842dfd9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_f89412d7-c975-4e45-b442-024c8a9c88b2" xlink:to="loc_us-gaap_SegmentDomain_7df33ddb-71c4-4e10-b7b7-99301842dfd9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityBrandsLightingAndLightingControlsSegmentMember_8eb8f62b-702b-4868-bf73-fe5f908df650" xlink:href="ayi-20231130.xsd#ayi_AcuityBrandsLightingAndLightingControlsSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_7df33ddb-71c4-4e10-b7b7-99301842dfd9" xlink:to="loc_ayi_AcuityBrandsLightingAndLightingControlsSegmentMember_8eb8f62b-702b-4868-bf73-fe5f908df650" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_IntelligentSpacesGroupSegmentMember_50fe4d9f-858a-41e4-a521-349e89b717f6" xlink:href="ayi-20231130.xsd#ayi_IntelligentSpacesGroupSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_7df33ddb-71c4-4e10-b7b7-99301842dfd9" xlink:to="loc_ayi_IntelligentSpacesGroupSegmentMember_50fe4d9f-858a-41e4-a521-349e89b717f6" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/DebtandLinesofCreditDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#DebtandLinesofCreditDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/DebtandLinesofCreditDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_41b2da23-9035-4bbf-8794-2a45bba67681" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_41b2da23-9035-4bbf-8794-2a45bba67681" xlink:to="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_fd32cc1c-f5ab-474b-b481-cff798a76b7a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_fd32cc1c-f5ab-474b-b481-cff798a76b7a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_17e5cebf-c9ef-470a-9b36-4982c81f7020" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_17e5cebf-c9ef-470a-9b36-4982c81f7020" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_DebtIssuancePricePercentageOfFaceValue_f1e7d1d7-85b3-4d89-8c9a-56b4a1c4375e" xlink:href="ayi-20231130.xsd#ayi_DebtIssuancePricePercentageOfFaceValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:to="loc_ayi_DebtIssuancePricePercentageOfFaceValue_f1e7d1d7-85b3-4d89-8c9a-56b4a1c4375e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredFinanceCostsGross_9066b5b9-ca09-4f78-8e62-81fbf69c3585" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DeferredFinanceCostsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:to="loc_us-gaap_DeferredFinanceCostsGross_9066b5b9-ca09-4f78-8e62-81fbf69c3585" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTerm_f027a281-f193-4608-96dc-c1919fd93251" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:to="loc_us-gaap_DebtInstrumentTerm_f027a281-f193-4608-96dc-c1919fd93251" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_cf9a77cb-6f15-432e-bb42-b5f197013d35" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_cf9a77cb-6f15-432e-bb42-b5f197013d35" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_LineOfCreditFacilityAccordionFeatureIncreaseDecreaseToBorrowingCapacity_1d33f739-2d4a-4453-abde-61abc2e5d04b" xlink:href="ayi-20231130.xsd#ayi_LineOfCreditFacilityAccordionFeatureIncreaseDecreaseToBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:to="loc_ayi_LineOfCreditFacilityAccordionFeatureIncreaseDecreaseToBorrowingCapacity_1d33f739-2d4a-4453-abde-61abc2e5d04b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermBorrowings_9526e021-7959-43da-8885-b6631851dd6e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShortTermBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:to="loc_us-gaap_ShortTermBorrowings_9526e021-7959-43da-8885-b6631851dd6e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity_c4ffb645-b6a3-420d-ac1f-4216a1247a24" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:to="loc_us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity_c4ffb645-b6a3-420d-ac1f-4216a1247a24" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LettersOfCreditOutstandingAmount_b9c236ce-6914-4e28-8a5d-9ef253f653fb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LettersOfCreditOutstandingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityAbstract_da5fc17e-31e4-4443-84aa-91d237a974a7" xlink:to="loc_us-gaap_LettersOfCreditOutstandingAmount_b9c236ce-6914-4e28-8a5d-9ef253f653fb" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_60858627-c737-4a07-a87f-027823dcf2ba" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_41b2da23-9035-4bbf-8794-2a45bba67681" xlink:to="loc_us-gaap_DebtInstrumentTable_60858627-c737-4a07-a87f-027823dcf2ba" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_0b543bd5-6651-447c-af26-1bb9a534e37f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_60858627-c737-4a07-a87f-027823dcf2ba" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_0b543bd5-6651-447c-af26-1bb9a534e37f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_0b543bd5-6651-447c-af26-1bb9a534e37f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_0b543bd5-6651-447c-af26-1bb9a534e37f" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_0b543bd5-6651-447c-af26-1bb9a534e37f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_d44ad056-17d8-4185-8154-d9e55618eb55" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_0b543bd5-6651-447c-af26-1bb9a534e37f" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_d44ad056-17d8-4185-8154-d9e55618eb55" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnsecuredDebtMember_a4791a31-ebca-43b3-aca8-a03216508664" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UnsecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_d44ad056-17d8-4185-8154-d9e55618eb55" xlink:to="loc_us-gaap_UnsecuredDebtMember_a4791a31-ebca-43b3-aca8-a03216508664" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_a2c582ab-8043-4e19-8da7-16b8df908326" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_d44ad056-17d8-4185-8154-d9e55618eb55" xlink:to="loc_us-gaap_LineOfCreditMember_a2c582ab-8043-4e19-8da7-16b8df908326" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LetterOfCreditMember_aca24387-7086-436b-bbc1-4a5ffc375ba2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LetterOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_d44ad056-17d8-4185-8154-d9e55618eb55" xlink:to="loc_us-gaap_LetterOfCreditMember_aca24387-7086-436b-bbc1-4a5ffc375ba2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_36ca11a8-f189-41b2-966c-bc5a34172b2f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_60858627-c737-4a07-a87f-027823dcf2ba" xlink:to="loc_us-gaap_DebtInstrumentAxis_36ca11a8-f189-41b2-966c-bc5a34172b2f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_36ca11a8-f189-41b2-966c-bc5a34172b2f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_36ca11a8-f189-41b2-966c-bc5a34172b2f" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_36ca11a8-f189-41b2-966c-bc5a34172b2f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_809fd288-4193-4a7b-94ca-8ee201eb5cb7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_36ca11a8-f189-41b2-966c-bc5a34172b2f" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_809fd288-4193-4a7b-94ca-8ee201eb5cb7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_A2150SeniorUnsecuredNotesDueDecember2030Member_4cf2eed3-9fe7-42e1-b3e2-bebe49ff5563" xlink:href="ayi-20231130.xsd#ayi_A2150SeniorUnsecuredNotesDueDecember2030Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_809fd288-4193-4a7b-94ca-8ee201eb5cb7" xlink:to="loc_ayi_A2150SeniorUnsecuredNotesDueDecember2030Member_4cf2eed3-9fe7-42e1-b3e2-bebe49ff5563" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_TheRevolvingCreditFacilityMember_0c9480ad-e07d-4240-9d50-9c36ffe78784" xlink:href="ayi-20231130.xsd#ayi_TheRevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_809fd288-4193-4a7b-94ca-8ee201eb5cb7" xlink:to="loc_ayi_TheRevolvingCreditFacilityMember_0c9480ad-e07d-4240-9d50-9c36ffe78784" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_8b65b08e-b2fb-47a1-92f7-82d1aed9e575" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_60858627-c737-4a07-a87f-027823dcf2ba" xlink:to="loc_dei_LegalEntityAxis_8b65b08e-b2fb-47a1-92f7-82d1aed9e575" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_8b65b08e-b2fb-47a1-92f7-82d1aed9e575_default" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_8b65b08e-b2fb-47a1-92f7-82d1aed9e575" xlink:to="loc_dei_EntityDomain_8b65b08e-b2fb-47a1-92f7-82d1aed9e575_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_83ef9755-4433-41ac-a814-9acd997560f9" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_8b65b08e-b2fb-47a1-92f7-82d1aed9e575" xlink:to="loc_dei_EntityDomain_83ef9755-4433-41ac-a814-9acd997560f9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityBrandsLightingIncMember_34b33d1c-87cd-4bb2-b1ed-53474edafa26" xlink:href="ayi-20231130.xsd#ayi_AcuityBrandsLightingIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_83ef9755-4433-41ac-a814-9acd997560f9" xlink:to="loc_ayi_AcuityBrandsLightingIncMember_34b33d1c-87cd-4bb2-b1ed-53474edafa26" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_188f7a36-4adc-449f-9e31-f0be5db35353" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_60858627-c737-4a07-a87f-027823dcf2ba" xlink:to="loc_us-gaap_CreditFacilityAxis_188f7a36-4adc-449f-9e31-f0be5db35353" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_188f7a36-4adc-449f-9e31-f0be5db35353_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_188f7a36-4adc-449f-9e31-f0be5db35353" xlink:to="loc_us-gaap_CreditFacilityDomain_188f7a36-4adc-449f-9e31-f0be5db35353_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_f9fc7658-99cc-4af6-972d-3fe7495ba0d4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_188f7a36-4adc-449f-9e31-f0be5db35353" xlink:to="loc_us-gaap_CreditFacilityDomain_f9fc7658-99cc-4af6-972d-3fe7495ba0d4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_5a143ccb-5567-4d5c-83ee-d2f87c16d7ab" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_f9fc7658-99cc-4af6-972d-3fe7495ba0d4" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_5a143ccb-5567-4d5c-83ee-d2f87c16d7ab" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/CommitmentsandContingenciesDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#CommitmentsandContingenciesDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/CommitmentsandContingenciesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesLineItems_1786a34e-0d97-4806-91a7-87e9bd428f59" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LossContingenciesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyNumberOfPlaintiffs_50c23f1c-25f7-439a-ac10-ed8392690dde" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LossContingencyNumberOfPlaintiffs"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_1786a34e-0d97-4806-91a7-87e9bd428f59" xlink:to="loc_us-gaap_LossContingencyNumberOfPlaintiffs_50c23f1c-25f7-439a-ac10-ed8392690dde" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesTable_9094b17a-af26-4f30-9c43-57021a230bc0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LossContingenciesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LossContingenciesLineItems_1786a34e-0d97-4806-91a7-87e9bd428f59" xlink:to="loc_us-gaap_LossContingenciesTable_9094b17a-af26-4f30-9c43-57021a230bc0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_106f8b37-8690-4d79-b2cd-edb2cf19bece" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LossContingenciesByNatureOfContingencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LossContingenciesTable_9094b17a-af26-4f30-9c43-57021a230bc0" xlink:to="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_106f8b37-8690-4d79-b2cd-edb2cf19bece" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyNatureDomain_106f8b37-8690-4d79-b2cd-edb2cf19bece_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LossContingencyNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_106f8b37-8690-4d79-b2cd-edb2cf19bece" xlink:to="loc_us-gaap_LossContingencyNatureDomain_106f8b37-8690-4d79-b2cd-edb2cf19bece_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyNatureDomain_c5547044-e5cc-48b0-8dc7-ba9840b3574e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LossContingencyNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_106f8b37-8690-4d79-b2cd-edb2cf19bece" xlink:to="loc_us-gaap_LossContingencyNatureDomain_c5547044-e5cc-48b0-8dc7-ba9840b3574e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_DataSecurityIncidentsMember_30b37bb2-4ed8-46db-b652-37e76cbf45a8" xlink:href="ayi-20231130.xsd#ayi_DataSecurityIncidentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingencyNatureDomain_c5547044-e5cc-48b0-8dc7-ba9840b3574e" xlink:to="loc_ayi_DataSecurityIncidentsMember_30b37bb2-4ed8-46db-b652-37e76cbf45a8" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/ChangesinStockholdersEquityDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#ChangesinStockholdersEquityDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/ChangesinStockholdersEquityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_d3de2181-ddff-48bb-8fd0-872b0993a3f0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d3de2181-ddff-48bb-8fd0-872b0993a3f0" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_59990d97-13a7-4fa5-96d5-29114fb860c0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:to="loc_us-gaap_SharesOutstanding_59990d97-13a7-4fa5-96d5-29114fb860c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_4cfa9b7e-ea76-4325-b0bf-cc4e7e2bbb11" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:to="loc_us-gaap_StockholdersEquity_4cfa9b7e-ea76-4325-b0bf-cc4e7e2bbb11" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_b5e7d965-b7ed-475f-8f89-5fa7345330f3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:to="loc_us-gaap_NetIncomeLoss_b5e7d965-b7ed-475f-8f89-5fa7345330f3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_eb71922f-1124-4c97-ab32-3200466e7631" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_eb71922f-1124-4c97-ab32-3200466e7631" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures_6ef02ad9-90d4-453d-991c-58d1b2b42c9f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures_6ef02ad9-90d4-453d-991c-58d1b2b42c9f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures_943b2815-c712-46af-84d4-c6f8bd50ff18" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures_943b2815-c712-46af-84d4-c6f8bd50ff18" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_7a2e895c-8492-44b9-a176-b093c7981e33" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_7a2e895c-8492-44b9-a176-b093c7981e33" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Dividends_669bd635-ab38-4578-8f6c-d45687319990" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Dividends"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:to="loc_us-gaap_Dividends_669bd635-ab38-4578-8f6c-d45687319990" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_ef941b69-7897-4955-a650-a33f3fd4ad4f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_ef941b69-7897-4955-a650-a33f3fd4ad4f" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodShares_e5ce292a-402a-4423-8ff8-4f4f6ff99a12" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockRepurchasedDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodShares_e5ce292a-402a-4423-8ff8-4f4f6ff99a12" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodValue_1889443b-124c-4473-b955-b9b66132738e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockRepurchasedDuringPeriodValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_de5f2e7f-4738-4801-b9bf-3e412d8b90bc" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodValue_1889443b-124c-4473-b955-b9b66132738e" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesOutstanding_dd129c52-c3d8-4951-86bf-1c8b425ff1e1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_93ad0062-b2c5-434d-bbb0-626a6f278de2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockDividendsPerShareCashPaid_0c9e4d20-3ede-4ced-8ffa-4cd916645320" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockDividendsPerShareCashPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_d3de2181-ddff-48bb-8fd0-872b0993a3f0" xlink:to="loc_us-gaap_CommonStockDividendsPerShareCashPaid_0c9e4d20-3ede-4ced-8ffa-4cd916645320" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_d0c60ecf-3964-43c4-b5d2-7dee0e2cde1d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_d3de2181-ddff-48bb-8fd0-872b0993a3f0" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_d0c60ecf-3964-43c4-b5d2-7dee0e2cde1d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_e78d533b-1c6c-4370-b43a-fe9c9b4fe017" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_d0c60ecf-3964-43c4-b5d2-7dee0e2cde1d" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_e78d533b-1c6c-4370-b43a-fe9c9b4fe017" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_e78d533b-1c6c-4370-b43a-fe9c9b4fe017_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_e78d533b-1c6c-4370-b43a-fe9c9b4fe017" xlink:to="loc_us-gaap_EquityComponentDomain_e78d533b-1c6c-4370-b43a-fe9c9b4fe017_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_62b435c3-4731-41ff-94ce-e5e39f1b220d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_e78d533b-1c6c-4370-b43a-fe9c9b4fe017" xlink:to="loc_us-gaap_EquityComponentDomain_62b435c3-4731-41ff-94ce-e5e39f1b220d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_6fb9a427-8505-403b-a629-5397b8d4a024" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_62b435c3-4731-41ff-94ce-e5e39f1b220d" xlink:to="loc_us-gaap_CommonStockMember_6fb9a427-8505-403b-a629-5397b8d4a024" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_5e27b7d0-25d8-470b-8348-8af6a7294efa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_62b435c3-4731-41ff-94ce-e5e39f1b220d" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_5e27b7d0-25d8-470b-8348-8af6a7294efa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_1e1beda9-c42f-434a-8c57-120b1837884d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_62b435c3-4731-41ff-94ce-e5e39f1b220d" xlink:to="loc_us-gaap_RetainedEarningsMember_1e1beda9-c42f-434a-8c57-120b1837884d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_f2f71ce4-4bbe-44ff-b1d7-90afa92c3360" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_62b435c3-4731-41ff-94ce-e5e39f1b220d" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_f2f71ce4-4bbe-44ff-b1d7-90afa92c3360" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonMember_a9934c6f-2d0f-4422-87c9-335fec9e6bbb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TreasuryStockCommonMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_62b435c3-4731-41ff-94ce-e5e39f1b220d" xlink:to="loc_us-gaap_TreasuryStockCommonMember_a9934c6f-2d0f-4422-87c9-335fec9e6bbb" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/RevenueRecognitionDisaggregatedRevenuesDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#RevenueRecognitionDisaggregatedRevenuesDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/RevenueRecognitionDisaggregatedRevenuesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_a6ff686b-9e4d-42bf-a24f-724e6aa7d377" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_194aea28-3a8b-49b9-8e3c-3e64a7c44858" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_a6ff686b-9e4d-42bf-a24f-724e6aa7d377" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_194aea28-3a8b-49b9-8e3c-3e64a7c44858" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_2022ad7c-2560-4671-ad7a-6d81e5e45366" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_a6ff686b-9e4d-42bf-a24f-724e6aa7d377" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_2022ad7c-2560-4671-ad7a-6d81e5e45366" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerSalesChannelAxis_50fbaf71-2809-4710-9cd3-9c430b49c554" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ContractWithCustomerSalesChannelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_2022ad7c-2560-4671-ad7a-6d81e5e45366" xlink:to="loc_us-gaap_ContractWithCustomerSalesChannelAxis_50fbaf71-2809-4710-9cd3-9c430b49c554" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerSalesChannelDomain_50fbaf71-2809-4710-9cd3-9c430b49c554_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ContractWithCustomerSalesChannelDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelAxis_50fbaf71-2809-4710-9cd3-9c430b49c554" xlink:to="loc_us-gaap_ContractWithCustomerSalesChannelDomain_50fbaf71-2809-4710-9cd3-9c430b49c554_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerSalesChannelDomain_6b34cc6d-c6ab-48f8-9682-78837df81df3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ContractWithCustomerSalesChannelDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelAxis_50fbaf71-2809-4710-9cd3-9c430b49c554" xlink:to="loc_us-gaap_ContractWithCustomerSalesChannelDomain_6b34cc6d-c6ab-48f8-9682-78837df81df3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_SalesChannelThroughIndependentSalesNetworkMember_c679ddce-cbb5-488b-89f1-b5706ab06062" xlink:href="ayi-20231130.xsd#ayi_SalesChannelThroughIndependentSalesNetworkMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelDomain_6b34cc6d-c6ab-48f8-9682-78837df81df3" xlink:to="loc_ayi_SalesChannelThroughIndependentSalesNetworkMember_c679ddce-cbb5-488b-89f1-b5706ab06062" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_SalesChannelThroughDirectSalesNetworkMember_eeb6b2a0-a41f-4aa0-9757-30bb7299b649" xlink:href="ayi-20231130.xsd#ayi_SalesChannelThroughDirectSalesNetworkMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelDomain_6b34cc6d-c6ab-48f8-9682-78837df81df3" xlink:to="loc_ayi_SalesChannelThroughDirectSalesNetworkMember_eeb6b2a0-a41f-4aa0-9757-30bb7299b649" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_SalesChannelThroughRetailMember_9a96e93a-9577-40f1-9348-7b9bbced0ada" xlink:href="ayi-20231130.xsd#ayi_SalesChannelThroughRetailMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelDomain_6b34cc6d-c6ab-48f8-9682-78837df81df3" xlink:to="loc_ayi_SalesChannelThroughRetailMember_9a96e93a-9577-40f1-9348-7b9bbced0ada" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_SalesChannelThroughCorporateAccountsNetworkMember_7dd2dad9-161d-435d-a630-b3088b7e3417" xlink:href="ayi-20231130.xsd#ayi_SalesChannelThroughCorporateAccountsNetworkMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelDomain_6b34cc6d-c6ab-48f8-9682-78837df81df3" xlink:to="loc_ayi_SalesChannelThroughCorporateAccountsNetworkMember_7dd2dad9-161d-435d-a630-b3088b7e3417" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_SalesChannelThroughOriginalEquipmentManufacturerAndOtherMember_baaa43f7-3435-4e71-8f88-787f73911a44" xlink:href="ayi-20231130.xsd#ayi_SalesChannelThroughOriginalEquipmentManufacturerAndOtherMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelDomain_6b34cc6d-c6ab-48f8-9682-78837df81df3" xlink:to="loc_ayi_SalesChannelThroughOriginalEquipmentManufacturerAndOtherMember_baaa43f7-3435-4e71-8f88-787f73911a44" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_0244617c-fb10-4939-b1db-047e966cbb48" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_2022ad7c-2560-4671-ad7a-6d81e5e45366" xlink:to="loc_srt_ConsolidationItemsAxis_0244617c-fb10-4939-b1db-047e966cbb48" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_0244617c-fb10-4939-b1db-047e966cbb48_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_0244617c-fb10-4939-b1db-047e966cbb48" xlink:to="loc_srt_ConsolidationItemsDomain_0244617c-fb10-4939-b1db-047e966cbb48_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_20e36511-ecb1-49fe-9bc0-8790a74c5bfc" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_0244617c-fb10-4939-b1db-047e966cbb48" xlink:to="loc_srt_ConsolidationItemsDomain_20e36511-ecb1-49fe-9bc0-8790a74c5bfc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingSegmentsMember_f81e5cd3-853e-4559-86ef-91128fffb0f0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_20e36511-ecb1-49fe-9bc0-8790a74c5bfc" xlink:to="loc_us-gaap_OperatingSegmentsMember_f81e5cd3-853e-4559-86ef-91128fffb0f0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntersegmentEliminationMember_6e82d16a-f71b-4c64-8e12-b71337a6f768" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IntersegmentEliminationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_20e36511-ecb1-49fe-9bc0-8790a74c5bfc" xlink:to="loc_us-gaap_IntersegmentEliminationMember_6e82d16a-f71b-4c64-8e12-b71337a6f768" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_9d9c43d9-3085-4d7a-b1b4-74a8d68d2486" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_2022ad7c-2560-4671-ad7a-6d81e5e45366" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_9d9c43d9-3085-4d7a-b1b4-74a8d68d2486" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_9d9c43d9-3085-4d7a-b1b4-74a8d68d2486_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_9d9c43d9-3085-4d7a-b1b4-74a8d68d2486" xlink:to="loc_us-gaap_SegmentDomain_9d9c43d9-3085-4d7a-b1b4-74a8d68d2486_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_ea50adde-0857-47a4-b65a-a42567e268f6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_9d9c43d9-3085-4d7a-b1b4-74a8d68d2486" xlink:to="loc_us-gaap_SegmentDomain_ea50adde-0857-47a4-b65a-a42567e268f6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityBrandsLightingAndLightingControlsSegmentMember_d554c75c-921e-4b3e-959d-ac682ae605e7" xlink:href="ayi-20231130.xsd#ayi_AcuityBrandsLightingAndLightingControlsSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_ea50adde-0857-47a4-b65a-a42567e268f6" xlink:to="loc_ayi_AcuityBrandsLightingAndLightingControlsSegmentMember_d554c75c-921e-4b3e-959d-ac682ae605e7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_IntelligentSpacesGroupSegmentMember_8d7b41a3-b7af-4e45-93b4-9ebd0fba7fb7" xlink:href="ayi-20231130.xsd#ayi_IntelligentSpacesGroupSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_ea50adde-0857-47a4-b65a-a42567e268f6" xlink:to="loc_ayi_IntelligentSpacesGroupSegmentMember_8d7b41a3-b7af-4e45-93b4-9ebd0fba7fb7" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/PensionPlansDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#PensionPlansDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/PensionPlansDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d650d285-829e-4b4d-bf5f-bb484c0f8c06" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_493a28ee-7249-4a79-9f9f-fb94eaa37b8f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d650d285-829e-4b4d-bf5f-bb484c0f8c06" xlink:to="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_493a28ee-7249-4a79-9f9f-fb94eaa37b8f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanServiceCost_26f9c957-0c98-48d7-b9d5-f948d1f13fa6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanServiceCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_493a28ee-7249-4a79-9f9f-fb94eaa37b8f" xlink:to="loc_us-gaap_DefinedBenefitPlanServiceCost_26f9c957-0c98-48d7-b9d5-f948d1f13fa6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanInterestCost_8bb8a089-f9dc-4a42-9a27-75b2f8bddaf2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanInterestCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_493a28ee-7249-4a79-9f9f-fb94eaa37b8f" xlink:to="loc_us-gaap_DefinedBenefitPlanInterestCost_8bb8a089-f9dc-4a42-9a27-75b2f8bddaf2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_e089dccd-82f2-4567-b833-19930955b1e0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_493a28ee-7249-4a79-9f9f-fb94eaa37b8f" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_e089dccd-82f2-4567-b833-19930955b1e0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmortizationOfPriorServiceCostCredit_0fbff2bf-8b06-4ea8-9be3-7555434aef43" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanAmortizationOfPriorServiceCostCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_493a28ee-7249-4a79-9f9f-fb94eaa37b8f" xlink:to="loc_us-gaap_DefinedBenefitPlanAmortizationOfPriorServiceCostCredit_0fbff2bf-8b06-4ea8-9be3-7555434aef43" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanActuarialGainLoss_5abdffbb-21c6-4321-8ad2-618285bdd6c8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanActuarialGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_493a28ee-7249-4a79-9f9f-fb94eaa37b8f" xlink:to="loc_us-gaap_DefinedBenefitPlanActuarialGainLoss_5abdffbb-21c6-4321-8ad2-618285bdd6c8" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_85a2f251-2c03-4faf-adb5-e90651b9df65" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_493a28ee-7249-4a79-9f9f-fb94eaa37b8f" xlink:to="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_85a2f251-2c03-4faf-adb5-e90651b9df65" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_2e925c3a-1ef1-46aa-b29b-386cccd4a4d4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_d650d285-829e-4b4d-bf5f-bb484c0f8c06" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_2e925c3a-1ef1-46aa-b29b-386cccd4a4d4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeAxis_d7370169-c7d4-409a-bf90-04d9dbe6833c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetirementPlanTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_2e925c3a-1ef1-46aa-b29b-386cccd4a4d4" xlink:to="loc_us-gaap_RetirementPlanTypeAxis_d7370169-c7d4-409a-bf90-04d9dbe6833c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_d7370169-c7d4-409a-bf90-04d9dbe6833c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_d7370169-c7d4-409a-bf90-04d9dbe6833c" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_d7370169-c7d4-409a-bf90-04d9dbe6833c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_de832308-6ad4-4396-ae69-9ec75dd81617" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_d7370169-c7d4-409a-bf90-04d9dbe6833c" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_de832308-6ad4-4396-ae69-9ec75dd81617" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionPlansDefinedBenefitMember_969a25d2-7cfe-4cf9-89dc-386b55cb69d7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PensionPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_de832308-6ad4-4396-ae69-9ec75dd81617" xlink:to="loc_us-gaap_PensionPlansDefinedBenefitMember_969a25d2-7cfe-4cf9-89dc-386b55cb69d7" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/SpecialChargesDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#SpecialChargesDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/SpecialChargesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCostAndReserveLineItems_76fd48e5-4040-4cfe-8c87-d80e54c650e1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestructuringCostAndReserveLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCharges_d676c2d2-39a7-44e8-a39f-53fac9fb7dec" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestructuringCharges"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_76fd48e5-4040-4cfe-8c87-d80e54c650e1" xlink:to="loc_us-gaap_RestructuringCharges_d676c2d2-39a7-44e8-a39f-53fac9fb7dec" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseImpairmentLoss_461bd96a-b9e1-41b9-8b77-ae3449bcc930" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingLeaseImpairmentLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_76fd48e5-4040-4cfe-8c87-d80e54c650e1" xlink:to="loc_us-gaap_OperatingLeaseImpairmentLoss_461bd96a-b9e1-41b9-8b77-ae3449bcc930" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_a501fa11-6939-4077-bdf9-2eb55ec69f32" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRestructuringAndRelatedCostsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RestructuringCostAndReserveLineItems_76fd48e5-4040-4cfe-8c87-d80e54c650e1" xlink:to="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_a501fa11-6939-4077-bdf9-2eb55ec69f32" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCostAndReserveAxis_d2df7bd3-6b80-4d3d-8e71-eeeeab9f0c42" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestructuringCostAndReserveAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRestructuringAndRelatedCostsTable_a501fa11-6939-4077-bdf9-2eb55ec69f32" xlink:to="loc_us-gaap_RestructuringCostAndReserveAxis_d2df7bd3-6b80-4d3d-8e71-eeeeab9f0c42" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfRestructuringDomain_d2df7bd3-6b80-4d3d-8e71-eeeeab9f0c42_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TypeOfRestructuringDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RestructuringCostAndReserveAxis_d2df7bd3-6b80-4d3d-8e71-eeeeab9f0c42" xlink:to="loc_us-gaap_TypeOfRestructuringDomain_d2df7bd3-6b80-4d3d-8e71-eeeeab9f0c42_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfRestructuringDomain_768af83f-17f5-40c0-aba0-f5204820209b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TypeOfRestructuringDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RestructuringCostAndReserveAxis_d2df7bd3-6b80-4d3d-8e71-eeeeab9f0c42" xlink:to="loc_us-gaap_TypeOfRestructuringDomain_768af83f-17f5-40c0-aba0-f5204820209b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_EmployeeSeveranceAndOtherCostsMember_a0bbffd1-7872-49de-b513-0ab8a8b27172" xlink:href="ayi-20231130.xsd#ayi_EmployeeSeveranceAndOtherCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TypeOfRestructuringDomain_768af83f-17f5-40c0-aba0-f5204820209b" xlink:to="loc_ayi_EmployeeSeveranceAndOtherCostsMember_a0bbffd1-7872-49de-b513-0ab8a8b27172" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/EarningsPerShareAntidilutiveSecuritiesExcludedfromEPSComputationDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#EarningsPerShareAntidilutiveSecuritiesExcludedfromEPSComputationDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/EarningsPerShareAntidilutiveSecuritiesExcludedfromEPSComputationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_7f825ff5-223e-437a-bd9d-eee46f70931a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_521c5121-2f59-4cb7-83c8-83a8b6644456" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_7f825ff5-223e-437a-bd9d-eee46f70931a" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_521c5121-2f59-4cb7-83c8-83a8b6644456" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_e1742f10-2459-4a38-8b0c-d6f275bd93db" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_7f825ff5-223e-437a-bd9d-eee46f70931a" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_e1742f10-2459-4a38-8b0c-d6f275bd93db" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_993ffadc-f99d-4690-8a50-14c7edb953ad" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_e1742f10-2459-4a38-8b0c-d6f275bd93db" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_993ffadc-f99d-4690-8a50-14c7edb953ad" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_993ffadc-f99d-4690-8a50-14c7edb953ad_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_993ffadc-f99d-4690-8a50-14c7edb953ad" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_993ffadc-f99d-4690-8a50-14c7edb953ad_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_f53ce0cb-f273-4e27-9b73-6a1ffaebafdd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_993ffadc-f99d-4690-8a50-14c7edb953ad" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_f53ce0cb-f273-4e27-9b73-6a1ffaebafdd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_4fd17eeb-71bf-4165-bf8d-b80cdac00fd3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_f53ce0cb-f273-4e27-9b73-6a1ffaebafdd" xlink:to="loc_us-gaap_EmployeeStockOptionMember_4fd17eeb-71bf-4165-bf8d-b80cdac00fd3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember_422b7712-e832-40db-9a6a-7db95218083b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_f53ce0cb-f273-4e27-9b73-6a1ffaebafdd" xlink:to="loc_us-gaap_PerformanceSharesMember_422b7712-e832-40db-9a6a-7db95218083b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockMember_a4e138cf-8123-4815-bcf9-ee81de6123ae" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestrictedStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_f53ce0cb-f273-4e27-9b73-6a1ffaebafdd" xlink:to="loc_us-gaap_RestrictedStockMember_a4e138cf-8123-4815-bcf9-ee81de6123ae" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/ComprehensiveIncomeChangesinComponentsofAccumulatedOtherComprehensiveIncomeLossItemsDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#ComprehensiveIncomeChangesinComponentsofAccumulatedOtherComprehensiveIncomeLossItemsDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/ComprehensiveIncomeChangesinComponentsofAccumulatedOtherComprehensiveIncomeLossItemsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_1938a9e3-da07-42cf-b393-02ee6cf3a472" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_d6c7cad1-d381-4abc-9abf-986e5b7143e2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AOCIAttributableToParentNetOfTaxRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_1938a9e3-da07-42cf-b393-02ee6cf3a472" xlink:to="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_d6c7cad1-d381-4abc-9abf-986e5b7143e2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_81601834-af59-425e-92d5-d7fcc072f817" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_d6c7cad1-d381-4abc-9abf-986e5b7143e2" xlink:to="loc_us-gaap_StockholdersEquity_81601834-af59-425e-92d5-d7fcc072f817" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax_6190fad3-d097-4369-b0f7-25daf6d3e23a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_d6c7cad1-d381-4abc-9abf-986e5b7143e2" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax_6190fad3-d097-4369-b0f7-25daf6d3e23a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax_800f3d81-acdd-45a9-b02c-0b30a697ba7c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_d6c7cad1-d381-4abc-9abf-986e5b7143e2" xlink:to="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax_800f3d81-acdd-45a9-b02c-0b30a697ba7c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_39cb6c62-8633-4591-9c94-f264f564ef88" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_d6c7cad1-d381-4abc-9abf-986e5b7143e2" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_39cb6c62-8633-4591-9c94-f264f564ef88" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_e05b3980-a1f0-4a82-9ca0-d9651ac0f658" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_2525f0ed-806e-4bc3-a5bf-f2e6d62e2d70" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_1938a9e3-da07-42cf-b393-02ee6cf3a472" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_2525f0ed-806e-4bc3-a5bf-f2e6d62e2d70" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_362e8e94-efb2-4c94-a5b2-95d5c2580ffa" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_2525f0ed-806e-4bc3-a5bf-f2e6d62e2d70" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_362e8e94-efb2-4c94-a5b2-95d5c2580ffa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_362e8e94-efb2-4c94-a5b2-95d5c2580ffa_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_362e8e94-efb2-4c94-a5b2-95d5c2580ffa" xlink:to="loc_us-gaap_EquityComponentDomain_362e8e94-efb2-4c94-a5b2-95d5c2580ffa_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_5129257f-7a24-4aad-9e80-8785a17cc1ce" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_362e8e94-efb2-4c94-a5b2-95d5c2580ffa" xlink:to="loc_us-gaap_EquityComponentDomain_5129257f-7a24-4aad-9e80-8785a17cc1ce" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_1f939725-620f-47b8-90fa-84864200fb81" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_5129257f-7a24-4aad-9e80-8785a17cc1ce" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_1f939725-620f-47b8-90fa-84864200fb81" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedTranslationAdjustmentMember_4a03732b-22a8-433a-97a2-fc451f4cf6e3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedTranslationAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_1f939725-620f-47b8-90fa-84864200fb81" xlink:to="loc_us-gaap_AccumulatedTranslationAdjustmentMember_4a03732b-22a8-433a-97a2-fc451f4cf6e3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_1c6b41d1-c55a-4320-aa3d-d5c2881caa5a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_1f939725-620f-47b8-90fa-84864200fb81" xlink:to="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_1c6b41d1-c55a-4320-aa3d-d5c2881caa5a" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/ComprehensiveIncomeTaxAmountsAllocatedtoComponentsofOtherComprehensiveLossDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#ComprehensiveIncomeTaxAmountsAllocatedtoComponentsofOtherComprehensiveLossDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/ComprehensiveIncomeTaxAmountsAllocatedtoComponentsofOtherComprehensiveLossDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_308a670a-e8a8-43d0-b307-f849f1281f41" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossBeforeTaxPortionAttributableToParent_75275c07-88dd-4c00-8ddf-501945fd18d0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossBeforeTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_308a670a-e8a8-43d0-b307-f849f1281f41" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossBeforeTaxPortionAttributableToParent_75275c07-88dd-4c00-8ddf-501945fd18d0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossTaxPortionAttributableToParent1_46619100-8990-4ec7-a3f0-ab409cc677ba" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossTaxPortionAttributableToParent1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_308a670a-e8a8-43d0-b307-f849f1281f41" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossTaxPortionAttributableToParent1_46619100-8990-4ec7-a3f0-ab409cc677ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_f520404d-ba60-4998-a182-aa42571a7c30" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_308a670a-e8a8-43d0-b307-f849f1281f41" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_f520404d-ba60-4998-a182-aa42571a7c30" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAociCurrentPeriodBeforeTaxAttributableToParent_5243ad44-99c9-4167-b488-0756057e7181" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReclassificationFromAociCurrentPeriodBeforeTaxAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_308a670a-e8a8-43d0-b307-f849f1281f41" xlink:to="loc_us-gaap_ReclassificationFromAociCurrentPeriodBeforeTaxAttributableToParent_5243ad44-99c9-4167-b488-0756057e7181" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAociCurrentPeriodTax_b3339cd3-3b54-4f7b-b98f-66a43615fddb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReclassificationFromAociCurrentPeriodTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_308a670a-e8a8-43d0-b307-f849f1281f41" xlink:to="loc_us-gaap_ReclassificationFromAociCurrentPeriodTax_b3339cd3-3b54-4f7b-b98f-66a43615fddb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent_9e78dd4b-be44-4fd8-9402-511f96b589d7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_308a670a-e8a8-43d0-b307-f849f1281f41" xlink:to="loc_us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent_9e78dd4b-be44-4fd8-9402-511f96b589d7" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_9ad7e149-d5b7-42d2-91c6-857e463b3745" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_308a670a-e8a8-43d0-b307-f849f1281f41" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_9ad7e149-d5b7-42d2-91c6-857e463b3745" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_ae5bcad0-0afd-47f2-ab0d-494f7ea28ca0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_9ad7e149-d5b7-42d2-91c6-857e463b3745" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_ae5bcad0-0afd-47f2-ab0d-494f7ea28ca0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_ae5bcad0-0afd-47f2-ab0d-494f7ea28ca0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_ae5bcad0-0afd-47f2-ab0d-494f7ea28ca0" xlink:to="loc_us-gaap_EquityComponentDomain_ae5bcad0-0afd-47f2-ab0d-494f7ea28ca0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_f8dc5612-c54f-4d23-81a4-45db4380e18c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_ae5bcad0-0afd-47f2-ab0d-494f7ea28ca0" xlink:to="loc_us-gaap_EquityComponentDomain_f8dc5612-c54f-4d23-81a4-45db4380e18c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedTranslationAdjustmentMember_052fe5f4-9ad7-48ea-b3f8-d8d85c69696b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedTranslationAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f8dc5612-c54f-4d23-81a4-45db4380e18c" xlink:to="loc_us-gaap_AccumulatedTranslationAdjustmentMember_052fe5f4-9ad7-48ea-b3f8-d8d85c69696b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentNetPriorServiceCostCreditMember_034cb3f8-0dbd-4595-92ca-653078e7fcf3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedDefinedBenefitPlansAdjustmentNetPriorServiceCostCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f8dc5612-c54f-4d23-81a4-45db4380e18c" xlink:to="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentNetPriorServiceCostCreditMember_034cb3f8-0dbd-4595-92ca-653078e7fcf3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentNetUnamortizedGainLossMember_7ed1260c-004d-4c89-a91a-e63b8db3d5e6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedDefinedBenefitPlansAdjustmentNetUnamortizedGainLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f8dc5612-c54f-4d23-81a4-45db4380e18c" xlink:to="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentNetUnamortizedGainLossMember_7ed1260c-004d-4c89-a91a-e63b8db3d5e6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_56b4e2b2-246e-41df-980b-65c8278e8ce7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_f8dc5612-c54f-4d23-81a4-45db4380e18c" xlink:to="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_56b4e2b2-246e-41df-980b-65c8278e8ce7" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/SegmentInformationOperatingSegmentDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#SegmentInformationOperatingSegmentDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/SegmentInformationOperatingSegmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_f5c1f5fe-7cac-40bf-ac5c-e01ce52abf60" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportableSegments_5c39e55e-86f4-4396-adaf-b6c2a34e903e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NumberOfReportableSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_f5c1f5fe-7cac-40bf-ac5c-e01ce52abf60" xlink:to="loc_us-gaap_NumberOfReportableSegments_5c39e55e-86f4-4396-adaf-b6c2a34e903e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCharges_489f91e9-f301-41fd-88b8-59b90ed1f6a9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestructuringCharges"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_f5c1f5fe-7cac-40bf-ac5c-e01ce52abf60" xlink:to="loc_us-gaap_RestructuringCharges_489f91e9-f301-41fd-88b8-59b90ed1f6a9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_aac600d7-043d-4656-a979-4629d1e16c14" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_f5c1f5fe-7cac-40bf-ac5c-e01ce52abf60" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_aac600d7-043d-4656-a979-4629d1e16c14" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_07948623-d5a8-4435-8f4c-779d916b27c2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_f5c1f5fe-7cac-40bf-ac5c-e01ce52abf60" xlink:to="loc_us-gaap_OperatingIncomeLoss_07948623-d5a8-4435-8f4c-779d916b27c2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_15b95f54-10cd-4eab-a362-bccba64a502e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_f5c1f5fe-7cac-40bf-ac5c-e01ce52abf60" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_15b95f54-10cd-4eab-a362-bccba64a502e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_04208f6b-75f9-4ea8-96c2-39db199fee63" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_15b95f54-10cd-4eab-a362-bccba64a502e" xlink:to="loc_srt_ConsolidationItemsAxis_04208f6b-75f9-4ea8-96c2-39db199fee63" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_04208f6b-75f9-4ea8-96c2-39db199fee63_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_04208f6b-75f9-4ea8-96c2-39db199fee63" xlink:to="loc_srt_ConsolidationItemsDomain_04208f6b-75f9-4ea8-96c2-39db199fee63_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_f9bafc04-201f-448a-bb44-32aceac3c6a6" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_04208f6b-75f9-4ea8-96c2-39db199fee63" xlink:to="loc_srt_ConsolidationItemsDomain_f9bafc04-201f-448a-bb44-32aceac3c6a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingSegmentsMember_18359fb7-b6f0-484b-bea3-93d7b3b2a740" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_f9bafc04-201f-448a-bb44-32aceac3c6a6" xlink:to="loc_us-gaap_OperatingSegmentsMember_18359fb7-b6f0-484b-bea3-93d7b3b2a740" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntersegmentEliminationMember_2d1413dc-0378-4eb3-b8a4-161d92691fa3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IntersegmentEliminationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_f9bafc04-201f-448a-bb44-32aceac3c6a6" xlink:to="loc_us-gaap_IntersegmentEliminationMember_2d1413dc-0378-4eb3-b8a4-161d92691fa3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateNonSegmentMember_ac04757c-551a-4603-8255-5369aa0b2b70" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CorporateNonSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_f9bafc04-201f-448a-bb44-32aceac3c6a6" xlink:to="loc_us-gaap_CorporateNonSegmentMember_ac04757c-551a-4603-8255-5369aa0b2b70" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_6dd0b67f-e36b-4eb5-91f6-fd8ccbd10aed" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_15b95f54-10cd-4eab-a362-bccba64a502e" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_6dd0b67f-e36b-4eb5-91f6-fd8ccbd10aed" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_6dd0b67f-e36b-4eb5-91f6-fd8ccbd10aed_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_6dd0b67f-e36b-4eb5-91f6-fd8ccbd10aed" xlink:to="loc_us-gaap_SegmentDomain_6dd0b67f-e36b-4eb5-91f6-fd8ccbd10aed_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_0e6d2654-263d-4a37-a934-97f132ee6c91" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_6dd0b67f-e36b-4eb5-91f6-fd8ccbd10aed" xlink:to="loc_us-gaap_SegmentDomain_0e6d2654-263d-4a37-a934-97f132ee6c91" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityBrandsLightingAndLightingControlsSegmentMember_06f0a67d-f97c-4e9b-81bc-f2c5fcb1e3a3" xlink:href="ayi-20231130.xsd#ayi_AcuityBrandsLightingAndLightingControlsSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_0e6d2654-263d-4a37-a934-97f132ee6c91" xlink:to="loc_ayi_AcuityBrandsLightingAndLightingControlsSegmentMember_06f0a67d-f97c-4e9b-81bc-f2c5fcb1e3a3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_IntelligentSpacesGroupSegmentMember_dfd28595-2e28-41ce-85c6-6117afe58a95" xlink:href="ayi-20231130.xsd#ayi_IntelligentSpacesGroupSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_0e6d2654-263d-4a37-a934-97f132ee6c91" xlink:to="loc_ayi_IntelligentSpacesGroupSegmentMember_dfd28595-2e28-41ce-85c6-6117afe58a95" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/SegmentReportingIncomeBeforeIncomeTaxesDetails" xlink:type="simple" xlink:href="ayi-20231130.xsd#SegmentReportingIncomeBeforeIncomeTaxesDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/SegmentReportingIncomeBeforeIncomeTaxesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_e77a0aa8-f31f-4d2e-b07a-8d41f3e69a62" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_859dc112-e8fe-46c5-915b-4553e828a482" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e77a0aa8-f31f-4d2e-b07a-8d41f3e69a62" xlink:to="loc_us-gaap_OperatingIncomeLoss_859dc112-e8fe-46c5-915b-4553e828a482" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRevenueExpenseNet_817841ad-751b-4b7d-ad19-e6224fc753f5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InterestRevenueExpenseNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e77a0aa8-f31f-4d2e-b07a-8d41f3e69a62" xlink:to="loc_us-gaap_InterestRevenueExpenseNet_817841ad-751b-4b7d-ad19-e6224fc753f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_f3f9f3e1-92a6-49a8-a162-d00d9c444182" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e77a0aa8-f31f-4d2e-b07a-8d41f3e69a62" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_f3f9f3e1-92a6-49a8-a162-d00d9c444182" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_188426cf-6c1b-4cc2-a68b-4171faa2a948" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e77a0aa8-f31f-4d2e-b07a-8d41f3e69a62" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_188426cf-6c1b-4cc2-a68b-4171faa2a948" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_b0fa5a05-3cf7-42da-a448-7e35188538a4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e77a0aa8-f31f-4d2e-b07a-8d41f3e69a62" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_b0fa5a05-3cf7-42da-a448-7e35188538a4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_15221d0a-2d58-440d-8479-4d28d05192b2" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_b0fa5a05-3cf7-42da-a448-7e35188538a4" xlink:to="loc_srt_ConsolidationItemsAxis_15221d0a-2d58-440d-8479-4d28d05192b2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_15221d0a-2d58-440d-8479-4d28d05192b2_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_15221d0a-2d58-440d-8479-4d28d05192b2" xlink:to="loc_srt_ConsolidationItemsDomain_15221d0a-2d58-440d-8479-4d28d05192b2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_2a326845-7b4c-40a7-88fe-3bd5f1415c6a" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_15221d0a-2d58-440d-8479-4d28d05192b2" xlink:to="loc_srt_ConsolidationItemsDomain_2a326845-7b4c-40a7-88fe-3bd5f1415c6a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingSegmentsMember_24d7bed3-6712-49cb-8b4a-7a2a86d27c1e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_2a326845-7b4c-40a7-88fe-3bd5f1415c6a" xlink:to="loc_us-gaap_OperatingSegmentsMember_24d7bed3-6712-49cb-8b4a-7a2a86d27c1e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateNonSegmentMember_98d8ddb7-fe07-4f9e-9c6b-9916b45eaf5b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CorporateNonSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_2a326845-7b4c-40a7-88fe-3bd5f1415c6a" xlink:to="loc_us-gaap_CorporateNonSegmentMember_98d8ddb7-fe07-4f9e-9c6b-9916b45eaf5b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_c2e00575-00bc-4850-9be7-327c3f14b7e6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_b0fa5a05-3cf7-42da-a448-7e35188538a4" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_c2e00575-00bc-4850-9be7-327c3f14b7e6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_c2e00575-00bc-4850-9be7-327c3f14b7e6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_c2e00575-00bc-4850-9be7-327c3f14b7e6" xlink:to="loc_us-gaap_SegmentDomain_c2e00575-00bc-4850-9be7-327c3f14b7e6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_69ad80d6-b4de-4a32-ae8b-1deebbd8cf94" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_c2e00575-00bc-4850-9be7-327c3f14b7e6" xlink:to="loc_us-gaap_SegmentDomain_69ad80d6-b4de-4a32-ae8b-1deebbd8cf94" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityBrandsLightingAndLightingControlsSegmentMember_f7822029-1629-4afc-8f58-77ab92b0a5a4" xlink:href="ayi-20231130.xsd#ayi_AcuityBrandsLightingAndLightingControlsSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_69ad80d6-b4de-4a32-ae8b-1deebbd8cf94" xlink:to="loc_ayi_AcuityBrandsLightingAndLightingControlsSegmentMember_f7822029-1629-4afc-8f58-77ab92b0a5a4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_IntelligentSpacesGroupSegmentMember_dbc09793-f5e2-46eb-a8b2-173cfb11f262" xlink:href="ayi-20231130.xsd#ayi_IntelligentSpacesGroupSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_69ad80d6-b4de-4a32-ae8b-1deebbd8cf94" xlink:to="loc_ayi_IntelligentSpacesGroupSegmentMember_dbc09793-f5e2-46eb-a8b2-173cfb11f262" xlink:type="arc" order="1"/>
  </link:definitionLink>
</link:linkbase>
