<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2025 Workiva-->
<!--r:cffbb5fc-5fe1-4ce4-98cf-8267c47782fd,g:71fbe18f-f9f8-4cdb-bdb3-0e22db701462-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/AcquisitionsNarrativeDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#AcquisitionsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/AcquisitionsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_e4011d0f-a62b-4dcf-9702-71af83c1c121" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_20bb54ef-a522-42f3-af3d-4d25cee090c2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_e4011d0f-a62b-4dcf-9702-71af83c1c121" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_20bb54ef-a522-42f3-af3d-4d25cee090c2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_61debe56-1379-4063-a282-debd25ab139e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_20bb54ef-a522-42f3-af3d-4d25cee090c2" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_61debe56-1379-4063-a282-debd25ab139e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_61debe56-1379-4063-a282-debd25ab139e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_61debe56-1379-4063-a282-debd25ab139e" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_61debe56-1379-4063-a282-debd25ab139e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_e6e91930-db73-411a-8947-1e209571e622" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_61debe56-1379-4063-a282-debd25ab139e" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_e6e91930-db73-411a-8947-1e209571e622" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_QSCLLCMember_201338b6-09b8-4db3-968a-e1982a3a20ae" xlink:href="ayi-20250228.xsd#ayi_QSCLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_e6e91930-db73-411a-8947-1e209571e622" xlink:to="loc_ayi_QSCLLCMember_201338b6-09b8-4db3-968a-e1982a3a20ae" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferred1_f4ac7676-00f2-4fa2-b872-0a7dd8aee214" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationConsiderationTransferred1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_e4011d0f-a62b-4dcf-9702-71af83c1c121" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferred1_f4ac7676-00f2-4fa2-b872-0a7dd8aee214" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_d274b23e-d334-4bd0-8e81-076d63d71086" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationAcquisitionRelatedCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_e4011d0f-a62b-4dcf-9702-71af83c1c121" xlink:to="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_d274b23e-d334-4bd0-8e81-076d63d71086" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionPurchasePriceAllocationGoodwillExpectedTaxDeductibleAmount_c4718338-d52c-43a8-8124-c4b06b13c42f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionPurchasePriceAllocationGoodwillExpectedTaxDeductibleAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_e4011d0f-a62b-4dcf-9702-71af83c1c121" xlink:to="loc_us-gaap_BusinessAcquisitionPurchasePriceAllocationGoodwillExpectedTaxDeductibleAmount_c4718338-d52c-43a8-8124-c4b06b13c42f" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/AcquisitionsScheduleofRecognizedIdentifiedAssetsAcquiredandLiabilitiesAssumedDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#AcquisitionsScheduleofRecognizedIdentifiedAssetsAcquiredandLiabilitiesAssumedDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/AcquisitionsScheduleofRecognizedIdentifiedAssetsAcquiredandLiabilitiesAssumedDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_5eea8710-96f2-4b41-98b3-e420aeb9bd46" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_5eea8710-96f2-4b41-98b3-e420aeb9bd46" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_8a7b780d-6b87-4e87-be89-f21bb4cbdf80" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_5eea8710-96f2-4b41-98b3-e420aeb9bd46" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_8a7b780d-6b87-4e87-be89-f21bb4cbdf80" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_8a7b780d-6b87-4e87-be89-f21bb4cbdf80_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_8a7b780d-6b87-4e87-be89-f21bb4cbdf80" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_8a7b780d-6b87-4e87-be89-f21bb4cbdf80_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_9fc93666-5ede-413e-bce4-0de3077ff050" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_8a7b780d-6b87-4e87-be89-f21bb4cbdf80" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_9fc93666-5ede-413e-bce4-0de3077ff050" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_QSCLLCMember_43030cf8-84a4-4349-ac4d-1115f31bda21" xlink:href="ayi-20250228.xsd#ayi_QSCLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_9fc93666-5ede-413e-bce4-0de3077ff050" xlink:to="loc_ayi_QSCLLCMember_43030cf8-84a4-4349-ac4d-1115f31bda21" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferred1_d1da808f-53cd-416c-bdb4-eb94d89f7518" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationConsiderationTransferred1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferred1_d1da808f-53cd-416c-bdb4-eb94d89f7518" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill_fa9b4435-1948-4d74-b12f-79491bdd937f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibleAssetsOtherThanGoodwill_fa9b4435-1948-4d74-b12f-79491bdd937f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_2c222ca4-57de-4cd4-aa3f-6f0bd19bad30" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInventory_2c222ca4-57de-4cd4-aa3f-6f0bd19bad30" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment_07dbf905-4e87-44e1-a73d-a8a80d1bb7fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment_07dbf905-4e87-44e1-a73d-a8a80d1bb7fc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOperatingLeaseAssets_2f416a69-13af-4ccf-8ea5-6aa688b4fe42" xlink:href="ayi-20250228.xsd#ayi_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOperatingLeaseAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_ayi_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOperatingLeaseAssets_2f416a69-13af-4ccf-8ea5-6aa688b4fe42" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables_73e931c1-d172-42b7-9ab8-bfcc5210c651" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables_73e931c1-d172-42b7-9ab8-bfcc5210c651" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsOther_a3ec9c8b-7c16-4517-8cdd-049b48dabab4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsOther_a3ec9c8b-7c16-4517-8cdd-049b48dabab4" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_ddd64d9c-0da7-4859-ad81-3d5c0ceb5ad2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_ddd64d9c-0da7-4859-ad81-3d5c0ceb5ad2" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable_0fa8c8fb-8032-4c3b-af08-6add69fae7ff" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable_0fa8c8fb-8032-4c3b-af08-6add69fae7ff" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesOperatingLeaseLiabilities_06e287ca-404a-47f1-82d9-e09c0d019ab6" xlink:href="ayi-20250228.xsd#ayi_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesOperatingLeaseLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_ayi_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesOperatingLeaseLiabilities_06e287ca-404a-47f1-82d9-e09c0d019ab6" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesOther_eb15952c-fd8c-4ac4-8f9e-1757782b396f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesOther_eb15952c-fd8c-4ac4-8f9e-1757782b396f" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilities_faf2843d-30a8-4c37-81e2-607e7cf644ee" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilities_faf2843d-30a8-4c37-81e2-607e7cf644ee" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_3b121b81-f0ef-460b-bb36-302ce5479498" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_3b121b81-f0ef-460b-bb36-302ce5479498" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_103b8845-8c44-412d-b35e-afd7881f1032" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_Goodwill_103b8845-8c44-412d-b35e-afd7881f1032" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsWeightedAverageUsefulLife_0c5f75ef-cb50-46ad-8037-54938622f9d3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AcquiredFiniteLivedIntangibleAssetsWeightedAverageUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_045136e0-58db-4ac0-8ed3-81597584c4f2" xlink:to="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsWeightedAverageUsefulLife_0c5f75ef-cb50-46ad-8037-54938622f9d3" xlink:type="arc" order="14"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/AcquisitionsScheduleoftheAmountofNetSalesandNetIncomeDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#AcquisitionsScheduleoftheAmountofNetSalesandNetIncomeDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/AcquisitionsScheduleoftheAmountofNetSalesandNetIncomeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_91efc853-8038-4c00-a994-ab5fcba30428" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_669178c2-ce13-45d5-b8e8-9793fb609c1f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_91efc853-8038-4c00-a994-ab5fcba30428" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_669178c2-ce13-45d5-b8e8-9793fb609c1f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_0768da82-c127-4bea-9465-502f08dd5182" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_669178c2-ce13-45d5-b8e8-9793fb609c1f" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_0768da82-c127-4bea-9465-502f08dd5182" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_0768da82-c127-4bea-9465-502f08dd5182_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_0768da82-c127-4bea-9465-502f08dd5182" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_0768da82-c127-4bea-9465-502f08dd5182_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_f9b20d30-14cc-44e2-8431-31b4589a3d49" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_0768da82-c127-4bea-9465-502f08dd5182" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_f9b20d30-14cc-44e2-8431-31b4589a3d49" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_QSCLLCMember_8d496212-2270-471b-8f99-2d05dd47583a" xlink:href="ayi-20250228.xsd#ayi_QSCLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_f9b20d30-14cc-44e2-8431-31b4589a3d49" xlink:to="loc_ayi_QSCLLCMember_8d496212-2270-471b-8f99-2d05dd47583a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonrecurringAdjustmentAxis_b9d22145-37d2-46ac-b54b-8c227972d52b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonrecurringAdjustmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_669178c2-ce13-45d5-b8e8-9793fb609c1f" xlink:to="loc_us-gaap_NonrecurringAdjustmentAxis_b9d22145-37d2-46ac-b54b-8c227972d52b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonrecurringAdjustmentDomain_b9d22145-37d2-46ac-b54b-8c227972d52b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonrecurringAdjustmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_NonrecurringAdjustmentAxis_b9d22145-37d2-46ac-b54b-8c227972d52b" xlink:to="loc_us-gaap_NonrecurringAdjustmentDomain_b9d22145-37d2-46ac-b54b-8c227972d52b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonrecurringAdjustmentDomain_00bff034-58ba-4f4d-aeb9-dcdc409b7434" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonrecurringAdjustmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_NonrecurringAdjustmentAxis_b9d22145-37d2-46ac-b54b-8c227972d52b" xlink:to="loc_us-gaap_NonrecurringAdjustmentDomain_00bff034-58ba-4f4d-aeb9-dcdc409b7434" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAdjustmentToInventoryMember_632692f3-170a-49ef-89d3-3210e402de97" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueAdjustmentToInventoryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonrecurringAdjustmentDomain_00bff034-58ba-4f4d-aeb9-dcdc409b7434" xlink:to="loc_us-gaap_FairValueAdjustmentToInventoryMember_632692f3-170a-49ef-89d3-3210e402de97" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationProFormaInformationRevenueOfAcquireeSinceAcquisitionDateActual_60f1899a-ea08-4ff0-b2e3-1d52a31a52d7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationProFormaInformationRevenueOfAcquireeSinceAcquisitionDateActual"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_91efc853-8038-4c00-a994-ab5fcba30428" xlink:to="loc_us-gaap_BusinessCombinationProFormaInformationRevenueOfAcquireeSinceAcquisitionDateActual_60f1899a-ea08-4ff0-b2e3-1d52a31a52d7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationProFormaInformationEarningsOrLossOfAcquireeSinceAcquisitionDateActual_5b77bcfd-018b-4c10-856e-a5f2713f7a83" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessCombinationProFormaInformationEarningsOrLossOfAcquireeSinceAcquisitionDateActual"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_91efc853-8038-4c00-a994-ab5fcba30428" xlink:to="loc_us-gaap_BusinessCombinationProFormaInformationEarningsOrLossOfAcquireeSinceAcquisitionDateActual_5b77bcfd-018b-4c10-856e-a5f2713f7a83" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_FiniteLivedIntangibleAssetsAcquiredAccumulatedAmortization_8a038091-bb9d-4dae-ba9f-525910907e50" xlink:href="ayi-20250228.xsd#ayi_FiniteLivedIntangibleAssetsAcquiredAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_91efc853-8038-4c00-a994-ab5fcba30428" xlink:to="loc_ayi_FiniteLivedIntangibleAssetsAcquiredAccumulatedAmortization_8a038091-bb9d-4dae-ba9f-525910907e50" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/AcquisitionsScheduleofBusinessAcquisitionProFormaInformationDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#AcquisitionsScheduleofBusinessAcquisitionProFormaInformationDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/AcquisitionsScheduleofBusinessAcquisitionProFormaInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_d9c09963-fa7f-4bcb-b204-c635fe37b33c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_c0b230cb-6fe5-48e5-b7a5-f2a8109a34d8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_d9c09963-fa7f-4bcb-b204-c635fe37b33c" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_c0b230cb-6fe5-48e5-b7a5-f2a8109a34d8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_d7e4613a-7b90-44e1-966a-95370cb97b6f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_c0b230cb-6fe5-48e5-b7a5-f2a8109a34d8" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_d7e4613a-7b90-44e1-966a-95370cb97b6f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_d7e4613a-7b90-44e1-966a-95370cb97b6f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_d7e4613a-7b90-44e1-966a-95370cb97b6f" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_d7e4613a-7b90-44e1-966a-95370cb97b6f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_3949eece-6079-4c0b-b79b-79e02298ce5c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_d7e4613a-7b90-44e1-966a-95370cb97b6f" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_3949eece-6079-4c0b-b79b-79e02298ce5c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_QSCLLCMember_b032a7b6-71e4-4294-9d97-2a338567f08e" xlink:href="ayi-20250228.xsd#ayi_QSCLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_3949eece-6079-4c0b-b79b-79e02298ce5c" xlink:to="loc_ayi_QSCLLCMember_b032a7b6-71e4-4294-9d97-2a338567f08e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonrecurringAdjustmentAxis_e37a785e-4b58-4c5d-ad7c-9ea1eb8e0eec" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonrecurringAdjustmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_c0b230cb-6fe5-48e5-b7a5-f2a8109a34d8" xlink:to="loc_us-gaap_NonrecurringAdjustmentAxis_e37a785e-4b58-4c5d-ad7c-9ea1eb8e0eec" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonrecurringAdjustmentDomain_e37a785e-4b58-4c5d-ad7c-9ea1eb8e0eec_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonrecurringAdjustmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_NonrecurringAdjustmentAxis_e37a785e-4b58-4c5d-ad7c-9ea1eb8e0eec" xlink:to="loc_us-gaap_NonrecurringAdjustmentDomain_e37a785e-4b58-4c5d-ad7c-9ea1eb8e0eec_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonrecurringAdjustmentDomain_1a488731-96d6-4cdf-ac89-1842ad62cab5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NonrecurringAdjustmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_NonrecurringAdjustmentAxis_e37a785e-4b58-4c5d-ad7c-9ea1eb8e0eec" xlink:to="loc_us-gaap_NonrecurringAdjustmentDomain_1a488731-96d6-4cdf-ac89-1842ad62cab5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAdjustmentToInventoryMember_8200c9f8-24f3-44bc-a404-5487066dc922" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueAdjustmentToInventoryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonrecurringAdjustmentDomain_1a488731-96d6-4cdf-ac89-1842ad62cab5" xlink:to="loc_us-gaap_FairValueAdjustmentToInventoryMember_8200c9f8-24f3-44bc-a404-5487066dc922" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AcquisitionRelatedCostsMember_7fb93a21-d9b3-4812-90ff-4f4555416cce" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AcquisitionRelatedCostsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NonrecurringAdjustmentDomain_1a488731-96d6-4cdf-ac89-1842ad62cab5" xlink:to="loc_us-gaap_AcquisitionRelatedCostsMember_7fb93a21-d9b3-4812-90ff-4f4555416cce" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionsProFormaRevenue_99678fa8-b3eb-4994-8896-bf5b5e223483" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionsProFormaRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_d9c09963-fa7f-4bcb-b204-c635fe37b33c" xlink:to="loc_us-gaap_BusinessAcquisitionsProFormaRevenue_99678fa8-b3eb-4994-8896-bf5b5e223483" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionsProFormaNetIncomeLoss_55b9db60-db88-4e88-9f28-3bb7f2ee4228" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BusinessAcquisitionsProFormaNetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_d9c09963-fa7f-4bcb-b204-c635fe37b33c" xlink:to="loc_us-gaap_BusinessAcquisitionsProFormaNetIncomeLoss_55b9db60-db88-4e88-9f28-3bb7f2ee4228" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/FairValueMeasurementsScheduleofCarryingValueandEstimatedFairValueofFinancialInstrumentsDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#FairValueMeasurementsScheduleofCarryingValueandEstimatedFairValueofFinancialInstrumentsDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/FairValueMeasurementsScheduleofCarryingValueandEstimatedFairValueofFinancialInstrumentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_de171c44-bb24-49a8-ba54-2f0cf915ea05" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_1c4a792d-3408-4fbb-babd-4c8c6182b2e9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_de171c44-bb24-49a8-ba54-2f0cf915ea05" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_1c4a792d-3408-4fbb-babd-4c8c6182b2e9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_3402b1aa-a985-4b21-8f95-68783791337c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_1c4a792d-3408-4fbb-babd-4c8c6182b2e9" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_3402b1aa-a985-4b21-8f95-68783791337c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3402b1aa-a985-4b21-8f95-68783791337c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_3402b1aa-a985-4b21-8f95-68783791337c" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_3402b1aa-a985-4b21-8f95-68783791337c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_bd304efc-bd8a-4c45-924a-8087ffa1ab1e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_3402b1aa-a985-4b21-8f95-68783791337c" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_bd304efc-bd8a-4c45-924a-8087ffa1ab1e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel12And3Member_a4edab93-36c9-4a9a-b880-1a1a1301e11b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueInputsLevel12And3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_bd304efc-bd8a-4c45-924a-8087ffa1ab1e" xlink:to="loc_us-gaap_FairValueInputsLevel12And3Member_a4edab93-36c9-4a9a-b880-1a1a1301e11b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_048f29ba-0b09-4215-a6fe-48a38b0dcd43" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueInputsLevel12And3Member_a4edab93-36c9-4a9a-b880-1a1a1301e11b" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_048f29ba-0b09-4215-a6fe-48a38b0dcd43" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_86fe0d71-cd2f-4dac-8886-fd186c67c3fd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueInputsLevel12And3Member_a4edab93-36c9-4a9a-b880-1a1a1301e11b" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_86fe0d71-cd2f-4dac-8886-fd186c67c3fd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_c9016d9e-8c51-4bfd-be70-4a415ad16103" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueInputsLevel12And3Member_a4edab93-36c9-4a9a-b880-1a1a1301e11b" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_c9016d9e-8c51-4bfd-be70-4a415ad16103" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasuredAtNetAssetValuePerShareMember_d29216ed-456e-444f-ae2b-b05baec66ee8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasuredAtNetAssetValuePerShareMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_bd304efc-bd8a-4c45-924a-8087ffa1ab1e" xlink:to="loc_us-gaap_FairValueMeasuredAtNetAssetValuePerShareMember_d29216ed-456e-444f-ae2b-b05baec66ee8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_b84c9bcf-1457-449a-acc5-c7be7d378e6e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_1c4a792d-3408-4fbb-babd-4c8c6182b2e9" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_b84c9bcf-1457-449a-acc5-c7be7d378e6e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_b84c9bcf-1457-449a-acc5-c7be7d378e6e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_b84c9bcf-1457-449a-acc5-c7be7d378e6e" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_b84c9bcf-1457-449a-acc5-c7be7d378e6e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_5594a398-1b03-449e-909f-deb3af0e99ed" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_b84c9bcf-1457-449a-acc5-c7be7d378e6e" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_5594a398-1b03-449e-909f-deb3af0e99ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_3fa12e5f-a3b5-4f16-8e28-c495aaf7ff78" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_5594a398-1b03-449e-909f-deb3af0e99ed" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_3fa12e5f-a3b5-4f16-8e28-c495aaf7ff78" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_1f62167e-8304-4943-bcf2-7b6e909fbb19" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_de171c44-bb24-49a8-ba54-2f0cf915ea05" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_1f62167e-8304-4943-bcf2-7b6e909fbb19" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_70be6eeb-09b8-4039-b62e-a3f451bd3d63" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_de171c44-bb24-49a8-ba54-2f0cf915ea05" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_70be6eeb-09b8-4039-b62e-a3f451bd3d63" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesWithoutReadilyDeterminableFairValueAmount_ce49c809-65a6-4394-9966-ec9fa6dbb408" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquitySecuritiesWithoutReadilyDeterminableFairValueAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_de171c44-bb24-49a8-ba54-2f0cf915ea05" xlink:to="loc_us-gaap_EquitySecuritiesWithoutReadilyDeterminableFairValueAmount_ce49c809-65a6-4394-9966-ec9fa6dbb408" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AssetFairValueDisclosureIncludingEquitySecuritiesWithoutReadilyDeterminableFairValueAmount_dc0258be-ac04-418b-adf8-9d87f2dd15fd" xlink:href="ayi-20250228.xsd#ayi_AssetFairValueDisclosureIncludingEquitySecuritiesWithoutReadilyDeterminableFairValueAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_de171c44-bb24-49a8-ba54-2f0cf915ea05" xlink:to="loc_ayi_AssetFairValueDisclosureIncludingEquitySecuritiesWithoutReadilyDeterminableFairValueAmount_dc0258be-ac04-418b-adf8-9d87f2dd15fd" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#FairValueMeasurementsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_5f379de4-ac45-4a0a-a2f6-0bb843af1f18" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_14538af7-0c64-4222-9209-4ff8bb5e519e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_5f379de4-ac45-4a0a-a2f6-0bb843af1f18" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_14538af7-0c64-4222-9209-4ff8bb5e519e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_b4bf5c82-3a3f-4b84-b806-2e4c2de49671" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_14538af7-0c64-4222-9209-4ff8bb5e519e" xlink:to="loc_us-gaap_DebtInstrumentAxis_b4bf5c82-3a3f-4b84-b806-2e4c2de49671" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_b4bf5c82-3a3f-4b84-b806-2e4c2de49671_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_b4bf5c82-3a3f-4b84-b806-2e4c2de49671" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_b4bf5c82-3a3f-4b84-b806-2e4c2de49671_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_31e5dfbf-a9ba-4cce-b9a3-80e69d9ca1bd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_b4bf5c82-3a3f-4b84-b806-2e4c2de49671" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_31e5dfbf-a9ba-4cce-b9a3-80e69d9ca1bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_A2150SeniorUnsecuredNotesDueDecember2030Member_38a664ec-5b08-41eb-aadf-a14cce339be4" xlink:href="ayi-20250228.xsd#ayi_A2150SeniorUnsecuredNotesDueDecember2030Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_31e5dfbf-a9ba-4cce-b9a3-80e69d9ca1bd" xlink:to="loc_ayi_A2150SeniorUnsecuredNotesDueDecember2030Member_38a664ec-5b08-41eb-aadf-a14cce339be4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_TheRevolvingCreditFacilityMember_6b2f66a4-7e6b-4e05-b72f-318a178cf468" xlink:href="ayi-20250228.xsd#ayi_TheRevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_31e5dfbf-a9ba-4cce-b9a3-80e69d9ca1bd" xlink:to="loc_ayi_TheRevolvingCreditFacilityMember_6b2f66a4-7e6b-4e05-b72f-318a178cf468" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_e30cf966-f91c-4738-9d53-f71f82dfa068" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_14538af7-0c64-4222-9209-4ff8bb5e519e" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_e30cf966-f91c-4738-9d53-f71f82dfa068" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_e30cf966-f91c-4738-9d53-f71f82dfa068_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_e30cf966-f91c-4738-9d53-f71f82dfa068" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_e30cf966-f91c-4738-9d53-f71f82dfa068_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_74915cbf-66d8-44f6-a05b-0d158f166321" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_e30cf966-f91c-4738-9d53-f71f82dfa068" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_74915cbf-66d8-44f6-a05b-0d158f166321" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_d1d0dcd0-9ae2-43cd-8347-56fbd4b5c669" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_74915cbf-66d8-44f6-a05b-0d158f166321" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_d1d0dcd0-9ae2-43cd-8347-56fbd4b5c669" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_98648db3-b534-4358-8430-e825f0e92927" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_d1d0dcd0-9ae2-43cd-8347-56fbd4b5c669" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_98648db3-b534-4358-8430-e825f0e92927" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_2d329210-4a12-4d14-be53-e005325255bc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_14538af7-0c64-4222-9209-4ff8bb5e519e" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_2d329210-4a12-4d14-be53-e005325255bc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_2d329210-4a12-4d14-be53-e005325255bc_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_2d329210-4a12-4d14-be53-e005325255bc" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_2d329210-4a12-4d14-be53-e005325255bc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_a7a99637-65e3-4992-8016-ce259c849bc4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_2d329210-4a12-4d14-be53-e005325255bc" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_a7a99637-65e3-4992-8016-ce259c849bc4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnsecuredDebtMember_faf422a9-6e9e-467e-9b81-65442f4af671" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnsecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_a7a99637-65e3-4992-8016-ce259c849bc4" xlink:to="loc_us-gaap_UnsecuredDebtMember_faf422a9-6e9e-467e-9b81-65442f4af671" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_7daf3305-4dab-40b1-b654-ccd902f73324" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_a7a99637-65e3-4992-8016-ce259c849bc4" xlink:to="loc_us-gaap_LineOfCreditMember_7daf3305-4dab-40b1-b654-ccd902f73324" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_48e56504-40d2-4a72-85cb-efda46b61b42" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_14538af7-0c64-4222-9209-4ff8bb5e519e" xlink:to="loc_us-gaap_CreditFacilityAxis_48e56504-40d2-4a72-85cb-efda46b61b42" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_48e56504-40d2-4a72-85cb-efda46b61b42_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_48e56504-40d2-4a72-85cb-efda46b61b42" xlink:to="loc_us-gaap_CreditFacilityDomain_48e56504-40d2-4a72-85cb-efda46b61b42_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_68cab2ac-e0c7-4244-894d-9214d9b38946" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_48e56504-40d2-4a72-85cb-efda46b61b42" xlink:to="loc_us-gaap_CreditFacilityDomain_68cab2ac-e0c7-4244-894d-9214d9b38946" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_882cb03a-2323-4687-9a40-c49d8210dfee" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_68cab2ac-e0c7-4244-894d-9214d9b38946" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_882cb03a-2323-4687-9a40-c49d8210dfee" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtFairValue_f3f5b2ed-7659-48da-916d-8124dcb5ad08" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongTermDebtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_5f379de4-ac45-4a0a-a2f6-0bb843af1f18" xlink:to="loc_us-gaap_LongTermDebtFairValue_f3f5b2ed-7659-48da-916d-8124dcb5ad08" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/PropertyPlantandEquipmentDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#PropertyPlantandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/PropertyPlantandEquipmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5445dd82-349f-4b55-8a5d-9d9c0cb11578" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_7c9e1c27-c069-4505-8a41-2d50345ee9e9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5445dd82-349f-4b55-8a5d-9d9c0cb11578" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_7c9e1c27-c069-4505-8a41-2d50345ee9e9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_74dafe4c-4587-49ab-a54e-2af72b2d850b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_7c9e1c27-c069-4505-8a41-2d50345ee9e9" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_74dafe4c-4587-49ab-a54e-2af72b2d850b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_74dafe4c-4587-49ab-a54e-2af72b2d850b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_74dafe4c-4587-49ab-a54e-2af72b2d850b" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_74dafe4c-4587-49ab-a54e-2af72b2d850b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_76708166-1ead-4ee0-8118-623fb8311aea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_74dafe4c-4587-49ab-a54e-2af72b2d850b" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_76708166-1ead-4ee0-8118-623fb8311aea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LandMember_37d79f5a-9a00-4f86-9545-089f16528098" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_76708166-1ead-4ee0-8118-623fb8311aea" xlink:to="loc_us-gaap_LandMember_37d79f5a-9a00-4f86-9545-089f16528098" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BuildingAndBuildingImprovementsMember_4499e45d-1261-4ee7-9cd0-4953d3bbf977" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BuildingAndBuildingImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_76708166-1ead-4ee0-8118-623fb8311aea" xlink:to="loc_us-gaap_BuildingAndBuildingImprovementsMember_4499e45d-1261-4ee7-9cd0-4953d3bbf977" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MachineryAndEquipmentMember_af611109-9b36-4f8a-a28d-19532fdfbc96" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_MachineryAndEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_76708166-1ead-4ee0-8118-623fb8311aea" xlink:to="loc_us-gaap_MachineryAndEquipmentMember_af611109-9b36-4f8a-a28d-19532fdfbc96" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_f68cad7e-9c98-4c38-98fe-2a2a74c3ebec" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5445dd82-349f-4b55-8a5d-9d9c0cb11578" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_f68cad7e-9c98-4c38-98fe-2a2a74c3ebec" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_6ae954cf-9151-4ee5-8ca9-985431f6d37f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5445dd82-349f-4b55-8a5d-9d9c0cb11578" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_6ae954cf-9151-4ee5-8ca9-985431f6d37f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_3739c9db-d620-49b3-bb7c-2be01170b039" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_5445dd82-349f-4b55-8a5d-9d9c0cb11578" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_3739c9db-d620-49b3-bb7c-2be01170b039" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/GoodwillandIntangibleAssetsScheduleofChangesinGoodwillDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#GoodwillandIntangibleAssetsScheduleofChangesinGoodwillDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/GoodwillandIntangibleAssetsScheduleofChangesinGoodwillDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_0343a9a4-3579-47b0-992d-58fb8d6b3a06" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable_103e58b9-03b4-4711-a9e5-c577a41f804c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_0343a9a4-3579-47b0-992d-58fb8d6b3a06" xlink:to="loc_us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable_103e58b9-03b4-4711-a9e5-c577a41f804c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_32ea0e3b-382b-410d-abf1-769056521c35" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable_103e58b9-03b4-4711-a9e5-c577a41f804c" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_32ea0e3b-382b-410d-abf1-769056521c35" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_32ea0e3b-382b-410d-abf1-769056521c35_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_32ea0e3b-382b-410d-abf1-769056521c35" xlink:to="loc_us-gaap_SegmentDomain_32ea0e3b-382b-410d-abf1-769056521c35_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_63f0343b-aa7c-4c91-8655-68ae1f2ec979" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_32ea0e3b-382b-410d-abf1-769056521c35" xlink:to="loc_us-gaap_SegmentDomain_63f0343b-aa7c-4c91-8655-68ae1f2ec979" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityBrandsLightingSegmentMember_fb440efb-c79f-495e-91d6-7c02549bb107" xlink:href="ayi-20250228.xsd#ayi_AcuityBrandsLightingSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_63f0343b-aa7c-4c91-8655-68ae1f2ec979" xlink:to="loc_ayi_AcuityBrandsLightingSegmentMember_fb440efb-c79f-495e-91d6-7c02549bb107" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityIntelligentSpacesSegmentMember_422dd482-45ff-40b0-89f3-07382795cfd8" xlink:href="ayi-20250228.xsd#ayi_AcuityIntelligentSpacesSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_63f0343b-aa7c-4c91-8655-68ae1f2ec979" xlink:to="loc_ayi_AcuityIntelligentSpacesSegmentMember_422dd482-45ff-40b0-89f3-07382795cfd8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillRollForward_326bf9b4-20bc-4db1-9842-50bbb187d957" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GoodwillRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_0343a9a4-3579-47b0-992d-58fb8d6b3a06" xlink:to="loc_us-gaap_GoodwillRollForward_326bf9b4-20bc-4db1-9842-50bbb187d957" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_160ae3ca-3867-437f-b632-65f4c7413ade" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_326bf9b4-20bc-4db1-9842-50bbb187d957" xlink:to="loc_us-gaap_Goodwill_160ae3ca-3867-437f-b632-65f4c7413ade" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillAcquiredDuringPeriod_8b54e3e2-9f30-4c7c-915c-a652d16f1bd9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GoodwillAcquiredDuringPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_326bf9b4-20bc-4db1-9842-50bbb187d957" xlink:to="loc_us-gaap_GoodwillAcquiredDuringPeriod_8b54e3e2-9f30-4c7c-915c-a652d16f1bd9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillForeignCurrencyTranslationGainLoss_0bdb2141-c01a-441b-831e-53513b6411c9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_GoodwillForeignCurrencyTranslationGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillRollForward_326bf9b4-20bc-4db1-9842-50bbb187d957" xlink:to="loc_us-gaap_GoodwillForeignCurrencyTranslationGainLoss_0bdb2141-c01a-441b-831e-53513b6411c9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_5b449ff4-e438-4cfe-9874-578064f3ca64" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Goodwill"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/DebtandLinesofCreditDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#DebtandLinesofCreditDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/DebtandLinesofCreditDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_648cce74-426c-49a8-885b-105007683200" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_DebtInstrumentTable_648cce74-426c-49a8-885b-105007683200" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_af3e575c-b866-4512-9430-d9325f630d3f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_648cce74-426c-49a8-885b-105007683200" xlink:to="loc_us-gaap_DebtInstrumentAxis_af3e575c-b866-4512-9430-d9325f630d3f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_af3e575c-b866-4512-9430-d9325f630d3f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_af3e575c-b866-4512-9430-d9325f630d3f" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_af3e575c-b866-4512-9430-d9325f630d3f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_00b79d06-3f16-4520-89fd-fdc58ae71d7e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_af3e575c-b866-4512-9430-d9325f630d3f" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_00b79d06-3f16-4520-89fd-fdc58ae71d7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_A2150SeniorUnsecuredNotesDueDecember2030Member_cc9a7d9f-d5fe-4991-9da1-f9f2639552fa" xlink:href="ayi-20250228.xsd#ayi_A2150SeniorUnsecuredNotesDueDecember2030Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_00b79d06-3f16-4520-89fd-fdc58ae71d7e" xlink:to="loc_ayi_A2150SeniorUnsecuredNotesDueDecember2030Member_cc9a7d9f-d5fe-4991-9da1-f9f2639552fa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_TheRevolvingCreditFacilityMember_eff2a24a-45d7-46e4-95ac-86d8d7611ebc" xlink:href="ayi-20250228.xsd#ayi_TheRevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_00b79d06-3f16-4520-89fd-fdc58ae71d7e" xlink:to="loc_ayi_TheRevolvingCreditFacilityMember_eff2a24a-45d7-46e4-95ac-86d8d7611ebc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_TermLoanFacilityMember_64a3a9b8-3948-4ed2-9eb3-8cbed10f1f90" xlink:href="ayi-20250228.xsd#ayi_TermLoanFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_00b79d06-3f16-4520-89fd-fdc58ae71d7e" xlink:to="loc_ayi_TermLoanFacilityMember_64a3a9b8-3948-4ed2-9eb3-8cbed10f1f90" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_TheCreditAgreementMember_0fd73d16-d576-4ed4-8557-fc35b3a8e120" xlink:href="ayi-20250228.xsd#ayi_TheCreditAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_00b79d06-3f16-4520-89fd-fdc58ae71d7e" xlink:to="loc_ayi_TheCreditAgreementMember_0fd73d16-d576-4ed4-8557-fc35b3a8e120" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_cad3eb03-bea5-495b-9a69-e2905e86077e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_648cce74-426c-49a8-885b-105007683200" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_cad3eb03-bea5-495b-9a69-e2905e86077e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_cad3eb03-bea5-495b-9a69-e2905e86077e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_cad3eb03-bea5-495b-9a69-e2905e86077e" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_cad3eb03-bea5-495b-9a69-e2905e86077e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_c5d795ff-4e29-406b-b5bc-f8b3e644a6bc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_cad3eb03-bea5-495b-9a69-e2905e86077e" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_c5d795ff-4e29-406b-b5bc-f8b3e644a6bc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnsecuredDebtMember_4410232e-3de3-43fb-8d17-aa819af2ab8b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_UnsecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_c5d795ff-4e29-406b-b5bc-f8b3e644a6bc" xlink:to="loc_us-gaap_UnsecuredDebtMember_4410232e-3de3-43fb-8d17-aa819af2ab8b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_5cc4c8b7-d63a-4651-ac89-aaa1c1c812f1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_c5d795ff-4e29-406b-b5bc-f8b3e644a6bc" xlink:to="loc_us-gaap_LineOfCreditMember_5cc4c8b7-d63a-4651-ac89-aaa1c1c812f1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LetterOfCreditMember_0750b83f-865a-4d1b-a337-1c27e3d9081b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LetterOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_c5d795ff-4e29-406b-b5bc-f8b3e644a6bc" xlink:to="loc_us-gaap_LetterOfCreditMember_0750b83f-865a-4d1b-a337-1c27e3d9081b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_55db7a61-f861-4359-9acf-1f3d68a331f5" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_648cce74-426c-49a8-885b-105007683200" xlink:to="loc_dei_LegalEntityAxis_55db7a61-f861-4359-9acf-1f3d68a331f5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_55db7a61-f861-4359-9acf-1f3d68a331f5_default" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_55db7a61-f861-4359-9acf-1f3d68a331f5" xlink:to="loc_dei_EntityDomain_55db7a61-f861-4359-9acf-1f3d68a331f5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_8e0fc96d-2dcd-477f-a2db-9f6a5bb0cc61" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_55db7a61-f861-4359-9acf-1f3d68a331f5" xlink:to="loc_dei_EntityDomain_8e0fc96d-2dcd-477f-a2db-9f6a5bb0cc61" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityBrandsLightingIncMember_ac7fc4c6-4ff2-44da-8fcb-83dae6210a52" xlink:href="ayi-20250228.xsd#ayi_AcuityBrandsLightingIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_8e0fc96d-2dcd-477f-a2db-9f6a5bb0cc61" xlink:to="loc_ayi_AcuityBrandsLightingIncMember_ac7fc4c6-4ff2-44da-8fcb-83dae6210a52" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_838f4470-1cf6-4a33-a6c5-5c9a52c37b7e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_648cce74-426c-49a8-885b-105007683200" xlink:to="loc_us-gaap_CreditFacilityAxis_838f4470-1cf6-4a33-a6c5-5c9a52c37b7e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_838f4470-1cf6-4a33-a6c5-5c9a52c37b7e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_838f4470-1cf6-4a33-a6c5-5c9a52c37b7e" xlink:to="loc_us-gaap_CreditFacilityDomain_838f4470-1cf6-4a33-a6c5-5c9a52c37b7e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_edc9edd3-2275-42cb-82b9-b23f560d7abe" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_838f4470-1cf6-4a33-a6c5-5c9a52c37b7e" xlink:to="loc_us-gaap_CreditFacilityDomain_edc9edd3-2275-42cb-82b9-b23f560d7abe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_a09c69c8-4c67-4304-80c3-eaf5217c2e0d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_edc9edd3-2275-42cb-82b9-b23f560d7abe" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_a09c69c8-4c67-4304-80c3-eaf5217c2e0d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_b2bb32a0-6305-4bcf-9ea8-b213858b5507" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_648cce74-426c-49a8-885b-105007683200" xlink:to="loc_srt_RangeAxis_b2bb32a0-6305-4bcf-9ea8-b213858b5507" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_b2bb32a0-6305-4bcf-9ea8-b213858b5507_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_b2bb32a0-6305-4bcf-9ea8-b213858b5507" xlink:to="loc_srt_RangeMember_b2bb32a0-6305-4bcf-9ea8-b213858b5507_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_5c0725ab-3d8d-48b9-81e4-a0855a3b8eac" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_b2bb32a0-6305-4bcf-9ea8-b213858b5507" xlink:to="loc_srt_RangeMember_5c0725ab-3d8d-48b9-81e4-a0855a3b8eac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_95613f8c-2d5f-42c8-b0c0-1d943808d907" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_5c0725ab-3d8d-48b9-81e4-a0855a3b8eac" xlink:to="loc_srt_MinimumMember_95613f8c-2d5f-42c8-b0c0-1d943808d907" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_76a01ec8-c593-4e56-bed9-9937313e0ee4" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_5c0725ab-3d8d-48b9-81e4-a0855a3b8eac" xlink:to="loc_srt_MaximumMember_76a01ec8-c593-4e56-bed9-9937313e0ee4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_281a40ee-9435-4684-a3f8-765d89a78476" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_648cce74-426c-49a8-885b-105007683200" xlink:to="loc_us-gaap_VariableRateAxis_281a40ee-9435-4684-a3f8-765d89a78476" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_281a40ee-9435-4684-a3f8-765d89a78476_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_281a40ee-9435-4684-a3f8-765d89a78476" xlink:to="loc_us-gaap_VariableRateDomain_281a40ee-9435-4684-a3f8-765d89a78476_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_5cb16935-6cce-40f3-b0ba-f57d306bd995" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_281a40ee-9435-4684-a3f8-765d89a78476" xlink:to="loc_us-gaap_VariableRateDomain_5cb16935-6cce-40f3-b0ba-f57d306bd995" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredOvernightFinancingRateSofrMember_5d2353cc-1e0f-4250-9046-d654650cd5df" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SecuredOvernightFinancingRateSofrMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_5cb16935-6cce-40f3-b0ba-f57d306bd995" xlink:to="loc_us-gaap_SecuredOvernightFinancingRateSofrMember_5d2353cc-1e0f-4250-9046-d654650cd5df" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BaseRateMember_6654dbd2-37c7-4240-b0eb-b9296ed7ba13" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_BaseRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_5cb16935-6cce-40f3-b0ba-f57d306bd995" xlink:to="loc_us-gaap_BaseRateMember_6654dbd2-37c7-4240-b0eb-b9296ed7ba13" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_42edead6-6619-410d-8fb5-c601c551a387" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_42edead6-6619-410d-8fb5-c601c551a387" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_d098f8c8-c171-40e1-9602-86f58cae7030" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_d098f8c8-c171-40e1-9602-86f58cae7030" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_DebtIssuancePricePercentageOfFaceValue_59abd655-962b-4d4c-93b9-48cf29c38863" xlink:href="ayi-20250228.xsd#ayi_DebtIssuancePricePercentageOfFaceValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_ayi_DebtIssuancePricePercentageOfFaceValue_59abd655-962b-4d4c-93b9-48cf29c38863" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredFinanceCostsGross_0a25954b-0c84-4719-9d8c-e32a1ff95ca2" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DeferredFinanceCostsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_DeferredFinanceCostsGross_0a25954b-0c84-4719-9d8c-e32a1ff95ca2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTerm_abebd0f8-93cb-4be9-8793-d93e70baf11c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_DebtInstrumentTerm_abebd0f8-93cb-4be9-8793-d93e70baf11c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_d9b9e0bb-6dcf-44c3-aa6b-369b6f16d8a4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_d9b9e0bb-6dcf-44c3-aa6b-369b6f16d8a4" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_LineOfCreditFacilityAccordionFeatureIncreaseDecreaseToBorrowingCapacity_04e68555-a683-4195-b45e-1a656cfd53ce" xlink:href="ayi-20250228.xsd#ayi_LineOfCreditFacilityAccordionFeatureIncreaseDecreaseToBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_ayi_LineOfCreditFacilityAccordionFeatureIncreaseDecreaseToBorrowingCapacity_04e68555-a683-4195-b45e-1a656cfd53ce" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermBorrowings_d4e9d0b1-3e2b-4f86-8248-b684bb3d50cb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ShortTermBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_ShortTermBorrowings_d4e9d0b1-3e2b-4f86-8248-b684bb3d50cb" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtLongtermAndShorttermCombinedAmount_099cf060-7cee-42c2-bc53-4b8962139993" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtLongtermAndShorttermCombinedAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_DebtLongtermAndShorttermCombinedAmount_099cf060-7cee-42c2-bc53-4b8962139993" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_DebtInstrumentRepaymentTerm_2d3e7edf-eec9-4b84-9e8b-5ad52a5ee5e2" xlink:href="ayi-20250228.xsd#ayi_DebtInstrumentRepaymentTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_ayi_DebtInstrumentRepaymentTerm_2d3e7edf-eec9-4b84-9e8b-5ad52a5ee5e2" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_8792ab95-9ea9-46e2-b59e-23815a0e83e4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_8792ab95-9ea9-46e2-b59e-23815a0e83e4" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityCommitmentFeePercentage_febfc59a-05d8-47ab-bed2-a63fa6227680" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LineOfCreditFacilityCommitmentFeePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_LineOfCreditFacilityCommitmentFeePercentage_febfc59a-05d8-47ab-bed2-a63fa6227680" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity_5bd35c50-1895-46d3-9f8f-83195e924b27" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_LineOfCreditFacilityCurrentBorrowingCapacity_5bd35c50-1895-46d3-9f8f-83195e924b27" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LettersOfCreditOutstandingAmount_9d6d08ce-432a-463a-af8a-755001e646ee" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_LettersOfCreditOutstandingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_260861ac-d4b7-420a-94b1-456099b746c5" xlink:to="loc_us-gaap_LettersOfCreditOutstandingAmount_9d6d08ce-432a-463a-af8a-755001e646ee" xlink:type="arc" order="13"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/ChangesinStockholdersEquityDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#ChangesinStockholdersEquityDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/ChangesinStockholdersEquityDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_1c2e50e9-62d9-446f-aa37-cd5ce645b6e8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_d6f17cf7-6750-4459-9f93-893477f5cd9b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_1c2e50e9-62d9-446f-aa37-cd5ce645b6e8" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_d6f17cf7-6750-4459-9f93-893477f5cd9b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_485db392-8d8c-42fa-a8c5-0e8f52069b9e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_d6f17cf7-6750-4459-9f93-893477f5cd9b" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_485db392-8d8c-42fa-a8c5-0e8f52069b9e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_485db392-8d8c-42fa-a8c5-0e8f52069b9e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_485db392-8d8c-42fa-a8c5-0e8f52069b9e" xlink:to="loc_us-gaap_EquityComponentDomain_485db392-8d8c-42fa-a8c5-0e8f52069b9e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_c4eb14b9-6f71-4c6b-90fd-fcd215dfec10" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_485db392-8d8c-42fa-a8c5-0e8f52069b9e" xlink:to="loc_us-gaap_EquityComponentDomain_c4eb14b9-6f71-4c6b-90fd-fcd215dfec10" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_8aab7391-6b01-481b-ad98-3aa3f9de67dd" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_c4eb14b9-6f71-4c6b-90fd-fcd215dfec10" xlink:to="loc_us-gaap_CommonStockMember_8aab7391-6b01-481b-ad98-3aa3f9de67dd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_715abc38-4442-4853-a9f8-bd780ddc4e38" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_c4eb14b9-6f71-4c6b-90fd-fcd215dfec10" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_715abc38-4442-4853-a9f8-bd780ddc4e38" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_0440c786-33b3-4923-89d8-20fd96042785" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_c4eb14b9-6f71-4c6b-90fd-fcd215dfec10" xlink:to="loc_us-gaap_RetainedEarningsMember_0440c786-33b3-4923-89d8-20fd96042785" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_deb6cfc6-0ab7-46c8-97da-996d5ed39b58" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_c4eb14b9-6f71-4c6b-90fd-fcd215dfec10" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_deb6cfc6-0ab7-46c8-97da-996d5ed39b58" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonMember_52dbd23f-c9ae-4ab3-b639-877b3d6be388" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_TreasuryStockCommonMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_c4eb14b9-6f71-4c6b-90fd-fcd215dfec10" xlink:to="loc_us-gaap_TreasuryStockCommonMember_52dbd23f-c9ae-4ab3-b639-877b3d6be388" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_1c2e50e9-62d9-446f-aa37-cd5ce645b6e8" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_b3bd2628-0f31-41bb-8c8d-198da3f772ea" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_b3bd2628-0f31-41bb-8c8d-198da3f772ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_8dcb6606-e07f-441d-8246-209c98353066" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_StockholdersEquity_8dcb6606-e07f-441d-8246-209c98353066" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_a4b8b048-95c9-48fe-8798-6ea06d3335b8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_NetIncomeLoss_a4b8b048-95c9-48fe-8798-6ea06d3335b8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_4032725a-5c57-4217-ad89-4b85dbedfd9d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_4032725a-5c57-4217-ad89-4b85dbedfd9d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures_9fe057a7-d102-4451-83c3-14b473f335f3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures_9fe057a7-d102-4451-83c3-14b473f335f3" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures_b8485d26-56a9-43c5-a5a2-c9511d4b6658" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures_b8485d26-56a9-43c5-a5a2-c9511d4b6658" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_58eb21ad-f7e6-4d06-8e45-18fa8cb0b9b8" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_58eb21ad-f7e6-4d06-8e45-18fa8cb0b9b8" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Dividends_61f5db9c-2ccf-4ff3-912d-0d9917fb3b3f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Dividends"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_Dividends_61f5db9c-2ccf-4ff3-912d-0d9917fb3b3f" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_07ab0fc2-f1fb-4add-90e5-05e840844953" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_07ab0fc2-f1fb-4add-90e5-05e840844953" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_73d5c8fb-e2f4-4013-ae90-056b2bee2516" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_73d5c8fb-e2f4-4013-ae90-056b2bee2516" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodShares_8e3546b8-4d10-4d40-b66e-ee8cb25557d6" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockRepurchasedDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodShares_8e3546b8-4d10-4d40-b66e-ee8cb25557d6" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodValue_e69c9959-286a-4109-ab44-083970836648" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockRepurchasedDuringPeriodValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5d8a6578-90cf-4a7c-b39f-a24a0f786f17" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodValue_e69c9959-286a-4109-ab44-083970836648" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_cc706ac3-eaf0-454e-8ba7-800446c55a64" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_dcba459c-0f6a-40b2-b0ef-6901c01ff230" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockDividendsPerShareCashPaid_1cbe4b4a-0bef-4ec2-8ef2-110db8fe180f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockDividendsPerShareCashPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_1c2e50e9-62d9-446f-aa37-cd5ce645b6e8" xlink:to="loc_us-gaap_CommonStockDividendsPerShareCashPaid_1cbe4b4a-0bef-4ec2-8ef2-110db8fe180f" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/RevenueScheduleofDisaggregatedRevenuesDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#RevenueScheduleofDisaggregatedRevenuesDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/RevenueScheduleofDisaggregatedRevenuesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_e299e56e-2510-4ec5-af8a-83fea9546575" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_305d2bbe-11d5-44f2-9033-dd16c676d46d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_e299e56e-2510-4ec5-af8a-83fea9546575" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_305d2bbe-11d5-44f2-9033-dd16c676d46d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_4f67c7a9-1238-4efc-b5b8-bc9f793a637e" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_305d2bbe-11d5-44f2-9033-dd16c676d46d" xlink:to="loc_srt_ConsolidationItemsAxis_4f67c7a9-1238-4efc-b5b8-bc9f793a637e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_4f67c7a9-1238-4efc-b5b8-bc9f793a637e_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_4f67c7a9-1238-4efc-b5b8-bc9f793a637e" xlink:to="loc_srt_ConsolidationItemsDomain_4f67c7a9-1238-4efc-b5b8-bc9f793a637e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_5f3636da-1710-434b-a676-3064412daa7c" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_4f67c7a9-1238-4efc-b5b8-bc9f793a637e" xlink:to="loc_srt_ConsolidationItemsDomain_5f3636da-1710-434b-a676-3064412daa7c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingSegmentsMember_8e071c68-c7c0-4b93-a334-42a883440f47" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_5f3636da-1710-434b-a676-3064412daa7c" xlink:to="loc_us-gaap_OperatingSegmentsMember_8e071c68-c7c0-4b93-a334-42a883440f47" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntersegmentEliminationMember_e953f7cf-8d9c-41ed-9d5a-90d8f1d4f77a" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IntersegmentEliminationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_5f3636da-1710-434b-a676-3064412daa7c" xlink:to="loc_us-gaap_IntersegmentEliminationMember_e953f7cf-8d9c-41ed-9d5a-90d8f1d4f77a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerSalesChannelAxis_e010b092-4e59-45f8-bc71-eab0e106dc22" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerSalesChannelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_305d2bbe-11d5-44f2-9033-dd16c676d46d" xlink:to="loc_us-gaap_ContractWithCustomerSalesChannelAxis_e010b092-4e59-45f8-bc71-eab0e106dc22" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerSalesChannelDomain_e010b092-4e59-45f8-bc71-eab0e106dc22_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerSalesChannelDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelAxis_e010b092-4e59-45f8-bc71-eab0e106dc22" xlink:to="loc_us-gaap_ContractWithCustomerSalesChannelDomain_e010b092-4e59-45f8-bc71-eab0e106dc22_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerSalesChannelDomain_aee4c41e-6e68-4be4-b1e9-402a08652b8f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ContractWithCustomerSalesChannelDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelAxis_e010b092-4e59-45f8-bc71-eab0e106dc22" xlink:to="loc_us-gaap_ContractWithCustomerSalesChannelDomain_aee4c41e-6e68-4be4-b1e9-402a08652b8f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_SalesChannelThroughIndependentSalesNetworkMember_5a189ed9-ff31-43aa-a1db-54c27faf720e" xlink:href="ayi-20250228.xsd#ayi_SalesChannelThroughIndependentSalesNetworkMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelDomain_aee4c41e-6e68-4be4-b1e9-402a08652b8f" xlink:to="loc_ayi_SalesChannelThroughIndependentSalesNetworkMember_5a189ed9-ff31-43aa-a1db-54c27faf720e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_SalesChannelThroughDirectSalesNetworkMember_e1750a1e-bc04-4cc0-8a07-0cbfc769ed27" xlink:href="ayi-20250228.xsd#ayi_SalesChannelThroughDirectSalesNetworkMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelDomain_aee4c41e-6e68-4be4-b1e9-402a08652b8f" xlink:to="loc_ayi_SalesChannelThroughDirectSalesNetworkMember_e1750a1e-bc04-4cc0-8a07-0cbfc769ed27" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_SalesChannelThroughRetailMember_b4c5cd1e-e6d4-4c4d-a160-5f1889256c5b" xlink:href="ayi-20250228.xsd#ayi_SalesChannelThroughRetailMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelDomain_aee4c41e-6e68-4be4-b1e9-402a08652b8f" xlink:to="loc_ayi_SalesChannelThroughRetailMember_b4c5cd1e-e6d4-4c4d-a160-5f1889256c5b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_SalesChannelThroughCorporateAccountsNetworkMember_5dff55ad-3769-47ed-a7bc-a528eb859a6a" xlink:href="ayi-20250228.xsd#ayi_SalesChannelThroughCorporateAccountsNetworkMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelDomain_aee4c41e-6e68-4be4-b1e9-402a08652b8f" xlink:to="loc_ayi_SalesChannelThroughCorporateAccountsNetworkMember_5dff55ad-3769-47ed-a7bc-a528eb859a6a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_SalesChannelThroughOriginalEquipmentManufacturerAndOtherMember_61722ba2-d144-42e4-b676-d3a6c769cb57" xlink:href="ayi-20250228.xsd#ayi_SalesChannelThroughOriginalEquipmentManufacturerAndOtherMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ContractWithCustomerSalesChannelDomain_aee4c41e-6e68-4be4-b1e9-402a08652b8f" xlink:to="loc_ayi_SalesChannelThroughOriginalEquipmentManufacturerAndOtherMember_61722ba2-d144-42e4-b676-d3a6c769cb57" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_780d248e-a4e0-4be7-bfec-817eb084ea55" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_305d2bbe-11d5-44f2-9033-dd16c676d46d" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_780d248e-a4e0-4be7-bfec-817eb084ea55" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_780d248e-a4e0-4be7-bfec-817eb084ea55_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_780d248e-a4e0-4be7-bfec-817eb084ea55" xlink:to="loc_us-gaap_SegmentDomain_780d248e-a4e0-4be7-bfec-817eb084ea55_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_606b7a70-c12c-47cb-b87d-f056585eae24" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_780d248e-a4e0-4be7-bfec-817eb084ea55" xlink:to="loc_us-gaap_SegmentDomain_606b7a70-c12c-47cb-b87d-f056585eae24" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityBrandsLightingSegmentMember_e4381149-f2b1-43c5-bbc2-e70db56ee022" xlink:href="ayi-20250228.xsd#ayi_AcuityBrandsLightingSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_606b7a70-c12c-47cb-b87d-f056585eae24" xlink:to="loc_ayi_AcuityBrandsLightingSegmentMember_e4381149-f2b1-43c5-bbc2-e70db56ee022" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityIntelligentSpacesSegmentMember_45ae37a5-5626-47b9-bda5-5ddffc8c3ec1" xlink:href="ayi-20250228.xsd#ayi_AcuityIntelligentSpacesSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_606b7a70-c12c-47cb-b87d-f056585eae24" xlink:to="loc_ayi_AcuityIntelligentSpacesSegmentMember_45ae37a5-5626-47b9-bda5-5ddffc8c3ec1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_843da1df-34aa-4f09-8e75-86f9a5d7c635" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_e299e56e-2510-4ec5-af8a-83fea9546575" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_843da1df-34aa-4f09-8e75-86f9a5d7c635" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/PensionPlansDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#PensionPlansDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/PensionPlansDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_f6b0da38-861e-4dd2-b211-0ae37b6c87fc" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_d1425e52-69f9-4b93-a743-0c5811901454" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_f6b0da38-861e-4dd2-b211-0ae37b6c87fc" xlink:to="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_d1425e52-69f9-4b93-a743-0c5811901454" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeAxis_dfb6b4e6-1dda-4e9c-8f58-5e28703dca04" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfDefinedBenefitPlansDisclosuresTable_d1425e52-69f9-4b93-a743-0c5811901454" xlink:to="loc_us-gaap_RetirementPlanTypeAxis_dfb6b4e6-1dda-4e9c-8f58-5e28703dca04" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_dfb6b4e6-1dda-4e9c-8f58-5e28703dca04_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_dfb6b4e6-1dda-4e9c-8f58-5e28703dca04" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_dfb6b4e6-1dda-4e9c-8f58-5e28703dca04_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetirementPlanTypeDomain_690cd5b3-cb74-4d1a-8b37-558dc85cbe6f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RetirementPlanTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RetirementPlanTypeAxis_dfb6b4e6-1dda-4e9c-8f58-5e28703dca04" xlink:to="loc_us-gaap_RetirementPlanTypeDomain_690cd5b3-cb74-4d1a-8b37-558dc85cbe6f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PensionPlansDefinedBenefitMember_4ba0acd8-981d-47f3-ab48-ffedd4756b83" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_PensionPlansDefinedBenefitMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RetirementPlanTypeDomain_690cd5b3-cb74-4d1a-8b37-558dc85cbe6f" xlink:to="loc_us-gaap_PensionPlansDefinedBenefitMember_4ba0acd8-981d-47f3-ab48-ffedd4756b83" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_3a905fdb-abfe-4490-a3c0-fdaa1c03f4c3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanDisclosureLineItems_f6b0da38-861e-4dd2-b211-0ae37b6c87fc" xlink:to="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_3a905fdb-abfe-4490-a3c0-fdaa1c03f4c3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanServiceCost_480c3987-42cf-4c9b-99d0-d27aa5c6e81f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanServiceCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_3a905fdb-abfe-4490-a3c0-fdaa1c03f4c3" xlink:to="loc_us-gaap_DefinedBenefitPlanServiceCost_480c3987-42cf-4c9b-99d0-d27aa5c6e81f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanInterestCost_28d998d6-8436-44be-9195-2dafacb8eceb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanInterestCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_3a905fdb-abfe-4490-a3c0-fdaa1c03f4c3" xlink:to="loc_us-gaap_DefinedBenefitPlanInterestCost_28d998d6-8436-44be-9195-2dafacb8eceb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_0f56af81-3fd1-4d61-8260-2835e42f1552" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_3a905fdb-abfe-4490-a3c0-fdaa1c03f4c3" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_0f56af81-3fd1-4d61-8260-2835e42f1552" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanActuarialGainLoss_fb5f06bf-5319-49d5-afd1-754fe3ec9c2d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanActuarialGainLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_3a905fdb-abfe-4490-a3c0-fdaa1c03f4c3" xlink:to="loc_us-gaap_DefinedBenefitPlanActuarialGainLoss_fb5f06bf-5319-49d5-afd1-754fe3ec9c2d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_f3d02452-9aa8-4da6-bc79-0bf5d0e8a102" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCostAbstract_3a905fdb-abfe-4490-a3c0-fdaa1c03f4c3" xlink:to="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_f3d02452-9aa8-4da6-bc79-0bf5d0e8a102" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/ComprehensiveIncomeScheduleofChangesinComponentsofAccumulatedOtherComprehensiveLossIncomeDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#ComprehensiveIncomeScheduleofChangesinComponentsofAccumulatedOtherComprehensiveLossIncomeDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/ComprehensiveIncomeScheduleofChangesinComponentsofAccumulatedOtherComprehensiveLossIncomeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_748f72af-3a05-4daf-8e3e-038e6f6b4ca3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_5d9dcc94-7dba-46d7-aa2b-03228eca88fb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_748f72af-3a05-4daf-8e3e-038e6f6b4ca3" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_5d9dcc94-7dba-46d7-aa2b-03228eca88fb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_295987de-23e9-4573-8080-c062b1502cb1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_5d9dcc94-7dba-46d7-aa2b-03228eca88fb" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_295987de-23e9-4573-8080-c062b1502cb1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_295987de-23e9-4573-8080-c062b1502cb1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_295987de-23e9-4573-8080-c062b1502cb1" xlink:to="loc_us-gaap_EquityComponentDomain_295987de-23e9-4573-8080-c062b1502cb1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_59caaa33-f99a-48da-8178-a21dfb17d788" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_295987de-23e9-4573-8080-c062b1502cb1" xlink:to="loc_us-gaap_EquityComponentDomain_59caaa33-f99a-48da-8178-a21dfb17d788" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_eee33e5a-ab5a-47e3-8e16-0667987ae57b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_59caaa33-f99a-48da-8178-a21dfb17d788" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_eee33e5a-ab5a-47e3-8e16-0667987ae57b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedTranslationAdjustmentMember_f40dd281-af16-4861-843c-702c884ff974" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedTranslationAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_eee33e5a-ab5a-47e3-8e16-0667987ae57b" xlink:to="loc_us-gaap_AccumulatedTranslationAdjustmentMember_f40dd281-af16-4861-843c-702c884ff974" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_e44c157c-2d0a-4c4e-aee4-052d92a842d1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_eee33e5a-ab5a-47e3-8e16-0667987ae57b" xlink:to="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_e44c157c-2d0a-4c4e-aee4-052d92a842d1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_9566f815-6ecb-4d89-b2d9-33407472390c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AOCIAttributableToParentNetOfTaxRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_748f72af-3a05-4daf-8e3e-038e6f6b4ca3" xlink:to="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_9566f815-6ecb-4d89-b2d9-33407472390c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_27c4352e-6ad6-461b-b0e0-1e19730c0068" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_9566f815-6ecb-4d89-b2d9-33407472390c" xlink:to="loc_us-gaap_StockholdersEquity_27c4352e-6ad6-461b-b0e0-1e19730c0068" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OciBeforeReclassificationsNetOfTaxAttributableToParent_cfff5752-7126-43ee-aaf5-094c55dd3e88" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OciBeforeReclassificationsNetOfTaxAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_9566f815-6ecb-4d89-b2d9-33407472390c" xlink:to="loc_us-gaap_OciBeforeReclassificationsNetOfTaxAttributableToParent_cfff5752-7126-43ee-aaf5-094c55dd3e88" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent_94f63e40-7547-43b9-86cb-b60bc383d2b9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_9566f815-6ecb-4d89-b2d9-33407472390c" xlink:to="loc_us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent_94f63e40-7547-43b9-86cb-b60bc383d2b9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_c4d1ff21-dc22-4ab7-94bc-835671f672b4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AOCIAttributableToParentNetOfTaxRollForward_9566f815-6ecb-4d89-b2d9-33407472390c" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_c4d1ff21-dc22-4ab7-94bc-835671f672b4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_cdce8670-c427-4a01-bcfa-000bd1376bc5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StockholdersEquity"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/ComprehensiveIncomeScheduleofTaxExpenseorBenefitAllocatedtoEachComponentofOtherComprehensiveLossDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#ComprehensiveIncomeScheduleofTaxExpenseorBenefitAllocatedtoEachComponentofOtherComprehensiveLossDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/ComprehensiveIncomeScheduleofTaxExpenseorBenefitAllocatedtoEachComponentofOtherComprehensiveLossDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_43ed9461-6ab3-499f-abe1-9680bb800c44" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_5954f410-3adc-40a3-98d6-843e619437eb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_43ed9461-6ab3-499f-abe1-9680bb800c44" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_5954f410-3adc-40a3-98d6-843e619437eb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_4be06e47-23d0-485f-89df-0d4573d21f59" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_5954f410-3adc-40a3-98d6-843e619437eb" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_4be06e47-23d0-485f-89df-0d4573d21f59" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_4be06e47-23d0-485f-89df-0d4573d21f59_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_4be06e47-23d0-485f-89df-0d4573d21f59" xlink:to="loc_us-gaap_EquityComponentDomain_4be06e47-23d0-485f-89df-0d4573d21f59_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_771ff113-1e58-4d45-bd12-7d5c97b62a64" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_4be06e47-23d0-485f-89df-0d4573d21f59" xlink:to="loc_us-gaap_EquityComponentDomain_771ff113-1e58-4d45-bd12-7d5c97b62a64" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedTranslationAdjustmentMember_b25b61f7-0932-4f56-82d4-4e6fb01247cb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedTranslationAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_771ff113-1e58-4d45-bd12-7d5c97b62a64" xlink:to="loc_us-gaap_AccumulatedTranslationAdjustmentMember_b25b61f7-0932-4f56-82d4-4e6fb01247cb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_02fba0e0-ac3f-4b10-90c5-c0c591e5605e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_771ff113-1e58-4d45-bd12-7d5c97b62a64" xlink:to="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_02fba0e0-ac3f-4b10-90c5-c0c591e5605e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossBeforeTaxPortionAttributableToParent_93961905-8091-4429-969a-335c78a0b551" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossBeforeTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_43ed9461-6ab3-499f-abe1-9680bb800c44" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossBeforeTaxPortionAttributableToParent_93961905-8091-4429-969a-335c78a0b551" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossTaxPortionAttributableToParent1_c0482767-40f0-4398-8f53-7a164acb98b1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossTaxPortionAttributableToParent1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_43ed9461-6ab3-499f-abe1-9680bb800c44" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossTaxPortionAttributableToParent1_c0482767-40f0-4398-8f53-7a164acb98b1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_af66eb5f-86da-4e8c-8f93-ad58038b6845" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_43ed9461-6ab3-499f-abe1-9680bb800c44" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_af66eb5f-86da-4e8c-8f93-ad58038b6845" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/SegmentInformationNarrativeDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#SegmentInformationNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/SegmentInformationNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_a8be8ef8-43ac-446b-9ee3-42eca8e2dc59" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_edef728a-8e27-48b6-b552-dcb45afe5b50" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_a8be8ef8-43ac-446b-9ee3-42eca8e2dc59" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_edef728a-8e27-48b6-b552-dcb45afe5b50" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_83a5f81a-eb11-42c6-8c1a-5b0106e87119" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_edef728a-8e27-48b6-b552-dcb45afe5b50" xlink:to="loc_srt_ConsolidationItemsAxis_83a5f81a-eb11-42c6-8c1a-5b0106e87119" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_83a5f81a-eb11-42c6-8c1a-5b0106e87119_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_83a5f81a-eb11-42c6-8c1a-5b0106e87119" xlink:to="loc_srt_ConsolidationItemsDomain_83a5f81a-eb11-42c6-8c1a-5b0106e87119_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_7ce73637-a564-43a4-a4e9-9505cebb3368" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_83a5f81a-eb11-42c6-8c1a-5b0106e87119" xlink:to="loc_srt_ConsolidationItemsDomain_7ce73637-a564-43a4-a4e9-9505cebb3368" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingSegmentsMember_364d9935-78ff-4cd3-8091-8730973d160e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_7ce73637-a564-43a4-a4e9-9505cebb3368" xlink:to="loc_us-gaap_OperatingSegmentsMember_364d9935-78ff-4cd3-8091-8730973d160e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_35ef8b9a-8b17-41ee-811b-8227b845292c" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_edef728a-8e27-48b6-b552-dcb45afe5b50" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_35ef8b9a-8b17-41ee-811b-8227b845292c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_35ef8b9a-8b17-41ee-811b-8227b845292c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_35ef8b9a-8b17-41ee-811b-8227b845292c" xlink:to="loc_us-gaap_SegmentDomain_35ef8b9a-8b17-41ee-811b-8227b845292c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_e5ad809c-0cb9-4d47-a2cb-712fdf2bcde1" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_35ef8b9a-8b17-41ee-811b-8227b845292c" xlink:to="loc_us-gaap_SegmentDomain_e5ad809c-0cb9-4d47-a2cb-712fdf2bcde1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityIntelligentSpacesSegmentMember_42565f5c-5058-4eef-a06f-881edbbccde4" xlink:href="ayi-20250228.xsd#ayi_AcuityIntelligentSpacesSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_e5ad809c-0cb9-4d47-a2cb-712fdf2bcde1" xlink:to="loc_ayi_AcuityIntelligentSpacesSegmentMember_42565f5c-5058-4eef-a06f-881edbbccde4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportableSegments_c9516792-644a-4ab0-831f-fc23f98e7099" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_NumberOfReportableSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_a8be8ef8-43ac-446b-9ee3-42eca8e2dc59" xlink:to="loc_us-gaap_NumberOfReportableSegments_c9516792-644a-4ab0-831f-fc23f98e7099" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_f00d6668-9b8b-41cf-945a-c377b3be62e4" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_Assets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_a8be8ef8-43ac-446b-9ee3-42eca8e2dc59" xlink:to="loc_us-gaap_Assets_f00d6668-9b8b-41cf-945a-c377b3be62e4" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/SegmentInformationScheduleofOperatingSegmentDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#SegmentInformationScheduleofOperatingSegmentDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/SegmentInformationScheduleofOperatingSegmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_fe0c6c14-0a7e-4ad5-9f61-e7c62cfd24ef" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_f7e5e097-7b1b-423a-8a52-000b71e7ad60" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fe0c6c14-0a7e-4ad5-9f61-e7c62cfd24ef" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_f7e5e097-7b1b-423a-8a52-000b71e7ad60" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_7862c3b4-dc34-4e99-9601-770f8eab1777" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_f7e5e097-7b1b-423a-8a52-000b71e7ad60" xlink:to="loc_srt_ConsolidationItemsAxis_7862c3b4-dc34-4e99-9601-770f8eab1777" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_7862c3b4-dc34-4e99-9601-770f8eab1777_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_7862c3b4-dc34-4e99-9601-770f8eab1777" xlink:to="loc_srt_ConsolidationItemsDomain_7862c3b4-dc34-4e99-9601-770f8eab1777_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_504f7cd6-f5c2-4a56-8154-4805d2417358" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_7862c3b4-dc34-4e99-9601-770f8eab1777" xlink:to="loc_srt_ConsolidationItemsDomain_504f7cd6-f5c2-4a56-8154-4805d2417358" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingSegmentsMember_9b71d041-3963-48ff-805c-3349a0ce5063" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_504f7cd6-f5c2-4a56-8154-4805d2417358" xlink:to="loc_us-gaap_OperatingSegmentsMember_9b71d041-3963-48ff-805c-3349a0ce5063" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntersegmentEliminationMember_8bfc3f91-634f-4749-8a06-f9dd2e49ce3e" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IntersegmentEliminationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_504f7cd6-f5c2-4a56-8154-4805d2417358" xlink:to="loc_us-gaap_IntersegmentEliminationMember_8bfc3f91-634f-4749-8a06-f9dd2e49ce3e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateNonSegmentMember_5de430e5-3d5b-42fa-8172-5789e2405a6b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CorporateNonSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_504f7cd6-f5c2-4a56-8154-4805d2417358" xlink:to="loc_us-gaap_CorporateNonSegmentMember_5de430e5-3d5b-42fa-8172-5789e2405a6b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_79740fc8-cbd1-4be8-ab5b-527df8eb79d5" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_f7e5e097-7b1b-423a-8a52-000b71e7ad60" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_79740fc8-cbd1-4be8-ab5b-527df8eb79d5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_79740fc8-cbd1-4be8-ab5b-527df8eb79d5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_79740fc8-cbd1-4be8-ab5b-527df8eb79d5" xlink:to="loc_us-gaap_SegmentDomain_79740fc8-cbd1-4be8-ab5b-527df8eb79d5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_bc081dbe-95db-4929-82b6-079f8aceb378" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_79740fc8-cbd1-4be8-ab5b-527df8eb79d5" xlink:to="loc_us-gaap_SegmentDomain_bc081dbe-95db-4929-82b6-079f8aceb378" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityBrandsLightingSegmentMember_50d221e0-d992-4378-a786-91d2f088a586" xlink:href="ayi-20250228.xsd#ayi_AcuityBrandsLightingSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_bc081dbe-95db-4929-82b6-079f8aceb378" xlink:to="loc_ayi_AcuityBrandsLightingSegmentMember_50d221e0-d992-4378-a786-91d2f088a586" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityIntelligentSpacesSegmentMember_20d3b7d2-ec8b-488d-8cc8-e97db2271258" xlink:href="ayi-20250228.xsd#ayi_AcuityIntelligentSpacesSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_bc081dbe-95db-4929-82b6-079f8aceb378" xlink:to="loc_ayi_AcuityIntelligentSpacesSegmentMember_20d3b7d2-ec8b-488d-8cc8-e97db2271258" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_c43e8bc1-887e-49ae-9a6c-b507a356a40f" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fe0c6c14-0a7e-4ad5-9f61-e7c62cfd24ef" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_c43e8bc1-887e-49ae-9a6c-b507a356a40f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_1727c055-96c7-456c-99c8-e1b0c8e866ad" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fe0c6c14-0a7e-4ad5-9f61-e7c62cfd24ef" xlink:to="loc_us-gaap_OperatingIncomeLoss_1727c055-96c7-456c-99c8-e1b0c8e866ad" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.acuitybrands.com/role/SegmentReportingScheduleofIncomeBeforeIncomeTaxesDetails" xlink:type="simple" xlink:href="ayi-20250228.xsd#SegmentReportingScheduleofIncomeBeforeIncomeTaxesDetails"/>
  <link:definitionLink xlink:role="http://www.acuitybrands.com/role/SegmentReportingScheduleofIncomeBeforeIncomeTaxesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_e18ee1a8-6aec-4521-95a3-859a296faa18" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_80a86c18-28af-4c53-b46b-c50a2feba172" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e18ee1a8-6aec-4521-95a3-859a296faa18" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_80a86c18-28af-4c53-b46b-c50a2feba172" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_2a343fc9-297c-429b-a84d-8d5b7fe65458" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_80a86c18-28af-4c53-b46b-c50a2feba172" xlink:to="loc_srt_ConsolidationItemsAxis_2a343fc9-297c-429b-a84d-8d5b7fe65458" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_2a343fc9-297c-429b-a84d-8d5b7fe65458_default" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_2a343fc9-297c-429b-a84d-8d5b7fe65458" xlink:to="loc_srt_ConsolidationItemsDomain_2a343fc9-297c-429b-a84d-8d5b7fe65458_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_60305dce-5a48-4ce2-9cb5-5a63c17a9280" xlink:href="https://xbrl.fasb.org/srt/2024/elts/srt-2024.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_2a343fc9-297c-429b-a84d-8d5b7fe65458" xlink:to="loc_srt_ConsolidationItemsDomain_60305dce-5a48-4ce2-9cb5-5a63c17a9280" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingSegmentsMember_82861cd7-7a5e-4316-9797-b4122fbd69cb" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_60305dce-5a48-4ce2-9cb5-5a63c17a9280" xlink:to="loc_us-gaap_OperatingSegmentsMember_82861cd7-7a5e-4316-9797-b4122fbd69cb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateNonSegmentMember_5871f64a-1378-48ba-9379-7d1d6c038bd3" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CorporateNonSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_60305dce-5a48-4ce2-9cb5-5a63c17a9280" xlink:to="loc_us-gaap_CorporateNonSegmentMember_5871f64a-1378-48ba-9379-7d1d6c038bd3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_7086b915-1bbe-439d-bdb8-01b2a3c5a0ec" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_80a86c18-28af-4c53-b46b-c50a2feba172" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_7086b915-1bbe-439d-bdb8-01b2a3c5a0ec" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_7086b915-1bbe-439d-bdb8-01b2a3c5a0ec_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_7086b915-1bbe-439d-bdb8-01b2a3c5a0ec" xlink:to="loc_us-gaap_SegmentDomain_7086b915-1bbe-439d-bdb8-01b2a3c5a0ec_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_d6c5b09f-dfed-4bd5-8658-71ff44ab0228" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_7086b915-1bbe-439d-bdb8-01b2a3c5a0ec" xlink:to="loc_us-gaap_SegmentDomain_d6c5b09f-dfed-4bd5-8658-71ff44ab0228" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityBrandsLightingSegmentMember_5ec02cee-3d36-400e-b6bc-a2fd29ae5f90" xlink:href="ayi-20250228.xsd#ayi_AcuityBrandsLightingSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_d6c5b09f-dfed-4bd5-8658-71ff44ab0228" xlink:to="loc_ayi_AcuityBrandsLightingSegmentMember_5ec02cee-3d36-400e-b6bc-a2fd29ae5f90" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ayi_AcuityIntelligentSpacesSegmentMember_df4ee9bb-8a49-4222-aedc-e13da1a1735a" xlink:href="ayi-20250228.xsd#ayi_AcuityIntelligentSpacesSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_d6c5b09f-dfed-4bd5-8658-71ff44ab0228" xlink:to="loc_ayi_AcuityIntelligentSpacesSegmentMember_df4ee9bb-8a49-4222-aedc-e13da1a1735a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_1e5cedfb-1706-498b-8716-486635cf708d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e18ee1a8-6aec-4521-95a3-859a296faa18" xlink:to="loc_us-gaap_OperatingIncomeLoss_1e5cedfb-1706-498b-8716-486635cf708d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRevenueExpenseNet_e2eb0f94-55d5-4b96-957f-9442ae04eb1b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_InterestRevenueExpenseNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e18ee1a8-6aec-4521-95a3-859a296faa18" xlink:to="loc_us-gaap_InterestRevenueExpenseNet_e2eb0f94-55d5-4b96-957f-9442ae04eb1b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_5d9b7f92-60a1-4478-b4db-5b0080864df7" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e18ee1a8-6aec-4521-95a3-859a296faa18" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_5d9b7f92-60a1-4478-b4db-5b0080864df7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_e4142b45-5de2-44ca-8055-12237a3567b9" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_e18ee1a8-6aec-4521-95a3-859a296faa18" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_e4142b45-5de2-44ca-8055-12237a3567b9" xlink:type="arc" order="3"/>
  </link:definitionLink>
</link:linkbase>
